Effects of the mixed probiotic microalgae-bacteria, Skeletonema costatum-Bacillus subtilis, on Stress tolerance and innate immunity prophenoloxidase (proPO) activating in Penaeus monodon larvae
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2014, Vol 4, Issue 5
Abstract
Shrimp farming like other industries, requires modern methods and techniques for increasing production yield. Novel technologies and other sciences such as biotechnology, nanotechnology and microbiology have important role in fishes aquaculture activities. Larvae of P. monodon were fed by conjugated B. subtilis and S. costatum with a high total protein rate. In this study tried to use a microalgea as a carrier for beneficial bacteria. These fed larvae by this carrier food showed responses to tolerance tests and prophenoloxidase I and II (proPO I & II) expression rates well in compare to the control samples. The analysis of immune-related gene expression of prophenoloxidase I and II of monodon post larvae were increased after being fed with 107 and 109 cellml-1 significantly. It is therefore said that 109 cellml-1 concentration of algae-bacteria can be very suitable for shrimp larva breeding. Because comparing the expression rates with control sample, proPO I and II genes of treated 109 cellml-1 sample were recorded 2.018 and 12.008 fold respectively.
Authors and Affiliations
Gholamreza Rafiee, Ahmad-ali Pourbabaee, Ali Agharokh, Mehran Avakhkeysami
Keywords
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