Effects of valproic acid (VPA) and levetiracetam (LEV) on proliferation, apoptosis and hormone secretion of the human choriocarcinoma BeWo cell line.
Journal Title: Pharmacological Reports - Year 2011, Vol 63, Issue 5
Abstract
Epilepsy has been associated with poor obstetric outcomes that may be the result of the epilepsy or a direct effect of anti-epileptic drugs on placentation. To investigate any direct effect of anti-epileptic drugs on cell proliferation, apoptosis and hormone secretion with focus on human chorionic gonadotropin (β-hCG), progesterone (P4) and 17β-estradiol (E2), BeWo cell line was cultured in the presence of different concentrations of sodium valproate (0.45, 0.6, 1.5 or 2 mM) or levetiracetam (0.07, 0.12, 0.3 or 0.5 mM) with appropriate solvent controls. Cell proliferation was measured using BrdU incorporation. Caspase-3 activity was used as a marker of cell apoptosis and was evaluated by a fluorometric assay. Additionally, hormone secretion was evaluated by ELISA kits. Dose-dependent action of VPA on cell proliferation occurred in parallel to stimulation of caspase-3 activity. LEV had no effect on cell proliferation, and after long term exposure to the drug, a decrease in caspase-3 activity was observed. A significant decrease in β-hCG, P4 and E2 production was observed when the cells were treated with VPA. LEV decreased β-hCG and E2 secretion but had no effect on P4 level. Direct inhibition of cell proliferation and hormone secretion along with apoptotic action suggest that exposure to VPA at therapeutic doses during early pregnancy should be approached with caution. Trophoblast cells appear to be less sensitive to LEV; however, further studies involving placental tissue are necessary to determine the safety of the drug.
Authors and Affiliations
Patrycja Kwiecińska, Justyna Wiśniewska, Ewa Gregoraszczuk
Keywords
Related Articles
- EP ID EP113901
- DOI -
- Views 84
- Downloads 0
Monocyte suppressing action of fenofibrate.
Since atherosclerosis has been proven to be an inflammatory disease, itis obvious that the proper treatment for dyslipidemia should not only correct lipid parameters but alsoinhibit inflammation. Monocytes and monocyte-d...
Early maternal separation: a rodent model of depression and a prevailing human condition.
The early life of most mammals is spent in close contact with the mother, and for the neonate, early maternal separation is a traumatic event that, depending on various conditions, may shape its behavioral and neurochemi...
Bisdemethoxycurcumin suppresses MCF-7 cells proliferation by inducing ROS accumulation and modulating senescence-related pathways.
Background: Bisdemethoxycurcumin (BDMC) is a natural derivative of curcumin present in the phenolic components extracted from the dried rhizome of Curcuma longa L. BDMC demonstrated potential chemotherapeutic activities...
Effect of p-isopropoxyphenylsuccinimide monohydrate on the anticonvulsant action of carbamazepine, phenobarbital, phenytoin and valproate in the mouse maximal electroshock-induced seizure model.
This study was designed to determine the effects of p-isopropoxyphenylsuccinimide monohydrate (IPPS) on the protective action of four classical antiepileptic drugs (carbamazepine, phenobarbital, phenytoin and valproate)...
Antioxidant treatment normalizes renal Na(+),K(+)-ATPase activity in leptin-treated rats.
Hyperleptinemia may be involved in the pathogenesis of obesity-associated hypertension, however, the mechanism of hypertensive effect of leptin is incompletely elucidated. Previously, we have demonstrated that chronic hy...