Efficient in vitro micropropagation of Gynura procumbens - an important rare medicinal plant, through shoot tip and nodal segment explants
Journal Title: Journal of Research in Biology - Year 2014, Vol 4, Issue 6
Abstract
Gynura procumbens is a medicinally important herbaceous plant species belonging to the family Asteraceae. It works against virus, inflammation and various types of allergies. It is used to treat rheumatic fever, migraine, kidney disease, diabetes, dysentery, various types of skin diseases and cancers. This study aimed to develop a suitable protocol for rapid production of Gynura procumbens from different explants. Shoot tip and nodal segment explants were used from one year mature plant. For shoot proliferation, among the two explants, shoot tips showed the best response (90%) on Murashige and Skoog (MS) medium supplemented with 1.0mg/l BAP and produced an average of 20±0.8 shootlets in each explants. In vitro derived shoots were subcultured on the similar medium and it gave similar production with healthy shoots. 100% rooting was observed on full strength MS medium containing NAA (0.5mg/l). Rooted plantlets were transferred for hardening into the mixture of soil, cowdung and sand (1:1:1). Then the rooted plantlets were successfully established in the field
Authors and Affiliations
Farhana Parvin, Jikrul Islam Md, Naoshin Jahan, Habiba Khan, Pallob Ebna Shaekh Md, Aminul Islam Md, Muhammed Hamidur Rahaman, Motiur Rahman Md
Keywords
Related Articles
- EP ID EP567174
- DOI -
- Views 85
- Downloads 0
Local people’s attitude towards conservation and development around Pichavaram mangrove ecosystem, Tamil Nadu, India
Studies in mangrove ecosystem are often focused on biological or ecological criteria and interdependency between mangroves and people is normally neglected. The situation is similar in Tamil Nadu; India which has a coast...
First report of Nereis (Neanthes) virens (Sars) an epitoky polychaete worm from Middle Strait, Baratang, Andaman Island, India
A sudden and mass outburst of the epitoky polychaete worm Nereis (Neanthes) virens (Sars)/ Alitta virens was observed of the surface waters of Middle Strait, Baratang, South Andaman Island during July 2014. This polychae...
Empirical validation of reliability of triangulation methods of mixed-method mode research: Quality improvement strategies for trypanosomiasis control
This paper presents a continuum of triangulation designs ranging from Participatory Rural Appraisal (PRA), surveys, parasitological to satellite data for a holistic approach to a research on trypanosomiasis – a disease a...
Studies on the identification of tomato rot fungi and physiological changes of tomato fruits infected with post-harvest fungi
Objectives: To detect post-harvest fungal attack on rotted tomato fruits and to determine different physiological changes in the contents of infected tomatoes. Material and methods: This study was carried out on various...
Evaluation of spherical agglomerated crystals of Lomefloxacin by IR and optical microscopy
The spherical crystallization technique was studied to improve the dissolution rate and bioavailability of lomefloxacin which is used as an antibacterial agent for Typhoid, Vaginal, GIT and ENT infection. In solvent chan...