Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2013, Vol 3, Issue 12
Abstract
A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92. Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars. The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1). The highest bud rooting of buds occurred on MS medium added with 1 mg/l NAA. The percentage of rooting varied from 16.66 to 75%. The highest percentage of survival plants after acclimatization were 41.73 and 57.63 for Beji and Maazoun respectively.
Authors and Affiliations
Rhimi Awatef, Boussaid Mohamed
Effects of some environmental factors on annual weed shepherd's purse (Capsella bursa-pastoris (L.) Medik.)
Shepherd’s purse (Capsella bursa-pastoris (L.) Medik.) is an annual weed species with a wide distribution in cereals of East Azarbaijan, Iran. Tow experiments were carried out based on randomised complete block design...
Spatial projection of dynamic modeling system: Exploration of Rubber tree plantation and the consequences of REDD+ for the populations of Toumodi in Côte d’Ivoire
This study aims at developing a model to investigate agricultural land change in the District of Toumodi due to the introduction of rubber tree and to evaluate the subsequent impact on the forests and the revenue of farm...
Investigation of land use change detection using satellite imagery (case study in Behbahan Province hills in Iran)
Change detection using remote sensing data has been intentioned much expansive with researchers in recent years. This study aimed to investigate changes in the area of Zagros forests using satellite imagery using TM and...
Reptile Diversity in Mt. Matutum Protected Landscape, South Cotabato, Philippines
Reptiles are highly diversewith high percentage of endemism in the Philippines. However, reptile diversity in Mindanao, the second largest island in the country,is poorly known. To determine the species richness, diversi...
Leaf flavonoids of Convolvulus L. species in Markazi Province, Iran
In order to study the leaf flavonoids pattern of Convolvulus leaves in 12 populations from four species (C. arvensis, C. commutatus, C. lineatus and C. pilosellaefolius) originated from Markazi Province, Iran, twodimens...