Electron Beam Irradiation of Resin Luting Agents - a Cytotoxic Evaluation on Dental Pulp Cells
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2014, Vol 4, Issue 19
Abstract
Aim: The aim of this study was to evaluate the cytotoxicity of three resin luting agents Rely X luting cement, Rely X luting 2 cement and Clearfil SA luting cement before and after electron beam irradiation. Materials and Methods: Growth and maintenance of cell cultures of human pulp cells was done in Dulbecco’s modified Eagle’s Medium (DMEM). The test samples were divided into two Categories: Irradiated Category and Non-radiated Category. Samples in Irradiated category were exposed to electron beam radiation at 200Gy. Three subgroups of radiated category and non radiated category were made. All the samples were subjected to MTT assay and spectrophotometric analysis and their cytotoxicity was assessed. Statistical analysis was done using t test. Results: Evaluation of Rely X luting Cement showed that radiated samples of powder, liquid and set cements showed decreased cell viability than non radiated samples. In Case of Rely X luting 2 cement, radiated samples showed increased cell viability for Paste A and Paste B samples. But in set material, irradiated samples showed decreased cell viability as compared to non radiated samples. For Clearfil SA luting Cement, Paste B showed increased cell viability for radiated samples. Paste A and Set cement of radiated samples showed decreased cell viability than non radiated samples. Conclusion: In the present study , the increased cytotoxicity of irradiated samples may be due to increase in the release of unbound monomers which may be due to chain breakage after irradiation and a reduction in the cytotoxicity which may be due to the cross linking of unbound monomers during irradiation.
Authors and Affiliations
Mithra N. Hegde, S. Shabin, Nidarsh D. Hegde, Shilpa S. Shetty, Suchetha Kumari, Ganesh Sanjeev
Keywords
Related Articles
- EP ID EP349315
- DOI 10.9734/BJMMR/2014/7762
- Views 68
- Downloads 0
Assessing the Influence of Exposure to Violent Living Condition, Knowledge, and Personal Motivation on Human Immunodeficiency Virus Infection Prevention Behavior among Historically Black College Students
Aim: This study assesses the effects of prevention education, prevention personal motivation, prevention knowledge, and past exposure to violent living conditions on human immunodeficiency virus (HIV) infection preventio...
Comparative Effects of Antiepileptic Agents Dichrostachys glomerata Ethanol Extract and Carbamazepine on Seizures and Anxiety in Mice
Aim: Treatment for neurological disorders have been sought through both herbal and orthodox medicine, therefore this present study compared the antiepileptic effects of Dichrostachys glomerata (D. glomerata) and Carbamaz...
Decitabine Induced Localized Maculopapular Eruption
Aims: Decitabine is a drug for the treatment of myelodysplastic syndromes and acute myeloid leukemia. It has a side-effect profile affecting many systems, including dermatologic side effects. Herein, we report a case wit...
Assessment of Individual Health of Russian Students from Different Social Groups
The assessment of individual health with the use of various techniques allows to obtain more complete information on the functioning of the body. For productive studying and professional development students need to have...
Anxiety Sensitivity and Comorbid Psychiatric Symptoms over the Course of Cognitive Behavioural Therapy for Panic Disorder
Aims: Anxiety sensitivity (AS) might be the core vulnerability in the pathogenesis of AXIS I diagnosis and might be the important transdiagnostic target for the interventions for comorbid psychiatric symptoms in panic di...