Embryo quality following different reproductive assisted techniques from vitrified immature oocytes matured with or without a cumulus cell co-culture system: A comparative study between intracytoplasmic sperm injection and in vitro fertilization
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 11
Abstract
To consider which methods of micromanipulation techniques increases the human oocyte maturation in vitro and fertilization rate cultured with or without CCs, we aimed to compare intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF). Five hundred fifty immature oocytes were retrieved and were randomly divided into two groups; oocytes that were cultured with CCs (Group A) and oocytes cultured without CCs (Group B). After in vitro maturation (IVM), only oocytes that displayed Metaphase II (M II) stage went randomly through the ICSI or IVF procedure. Maturation and fertilization rates were all examined. The mean age, basal follicle-stimulating hormone (FSH), and number of oocytes recovered for the patients were all comparable between the two study groups. The number of oocytes that reached M II (mature oocytes) was 194 out of 275in group A(CC-co cultured group) compared to 165/275 in group B (P = 0.009). The fertilization rates of matured human oocytes cultured with and without CCs by ICSI procedure was significantly higher than IVF method (P = 0.005 vs. P = 0.001, respectively). Findings of the current study revealed that the fertilization rate of in vitro matured oocytes during ICSI procedure is higher than IVF method.
Authors and Affiliations
Maryam Davari| Research Center, Department of Obstetrics and Gynecology, Jahrom University of Medical Sciences, Jahrom, Iran, Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran, Masih Allah Shakeri| Department of Internal medicine, Jahrom University of Medical Sciences, Jahrom, Iran, Pejman Hamedi Asl| Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran, Mina Shariati| Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran, Mohamed Amin Ghobadifar| Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran, Hossein Kargar Jahromi| Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran, Navid Kalani| Department of Internal medicine, Jahrom University of Medical Sciences, Jahrom, Iran, Farideh Mogharab| Research Center, Department of Obstetrics and Gynecology, Jahrom University of Medical Sciences, Jahrom, Iran, Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
Keywords
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