ENDOPHYTES FROM THE AQUATIC PLANT NELUMBO NUCIFERA: DIVERSITY PROFILE AND ACTIVITY CHARACTERIZATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 1
Abstract
Objective: Endophytes represent a niche habitat for the study of novel bio-and chemo diversity. Nelumbo nucifera is an aquatic plant that has not been characterized for endophyte diversity. This study was undertaken with the objective of isolating endophytes from submerged and aerial part of N. nucifera, study the diversity profile of the isolated endophytes and their antimicrobial, antioxidant, and siderophore production capacity.Methods: Endophytes were isolated from aerial and submerged parts of N. nucifera on different media (Starch Casein Nitrate, Glucose Yeast Extract, Nutrient and Potato Dextrose agar). These were further characterized for morphology (colony characteristics, Gram reaction), physiological characteristics (carbon, nitrogen utilization) and activity (antimicrobial, antioxidant, siderophore production). After dereplication, twelve isolates were studied further.Results: All endophyte isolates were Gram-positive bacteria, and one was a fungus. Isolate L-300 showed the highest antioxidant capacity (238 AAE g FW-1) and L-201 least (10 AAE g FW-1. Antimicrobial activity was exhibited against bacteria and fungal targets, with 50% endophytes active against both bacteria and fungi. Isolates L-003 and L-207 exhibited activity against Gram-negative clinical isolates as also fungi. Siderophore production was shown by 58% isolates with L-208 showing maximum activity.Conclusion: This is the first report on profiling of endophytes from N. nucifera. Results show that aquatic plants harbor diverse microbial population. Many promising isolates (such as L-003, L-211, L-214 and L-300) have been characterized in this study and results obtained of antioxidant, antimicrobial and siderophore production capacity demonstrate further utility in polypharmacological studies for identifying compounds of pharmaceutical and other industrial interest.Â
Authors and Affiliations
Tanuja Ijarwal, Bharti Sharma, Faiza Khan, A. Ibeyaima, Anuj Dwivedi, Narendra Saini, Indira P. Sarethy
Keywords
Related Articles
- EP ID EP577951
- DOI -
- Views 73
- Downloads 0
AB-FUBINACA, A SYNTHETIC CANNABINOID IN “FUNKY GREEN STUFFâ„¢â€
Objective: The objective of this research was to isolate and identify any potential synthetic cannabinoids disguised in the commercially available claimed-to-be herbal scent, known as “Funky Green Stuffâ„¢â€.Methods:...
PHARMACOLOGICAL EVALUATION OF HYDROALCOHOL AND CHLOROFORM EXTRACTS OF NYCTANTHES ARBOUR-TRISTIS L. FOR ANTIOXIDANT, ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY
Objective: The study was designed to evaluate the anti-oxidant, anti-inflammatory and analgesic activity of hydroalcohol (HA) and chloroform (CH) extracts of the whole plant of Nyctanthes Arbour-tristis L in laboratory a...
ANTI-MRSA OF THE ETHYL ACETATE CRUDE EXTRACT FROM LASIODIPLODIA PSEUDOTHEOBROMAE IBRL OS-64, AN ENDOPHYTIC FUNGUS ISOLATED FROM LEAF OF OCIMUM SANCTUM LINN
Objective: To investigate the effects of ethyl acetate crude extract of an endophytic fungus, L. pseudotheobromae IBRL OS-64 which was isolated from leaf of Ocimum sanctum against the growth of methicillin-resistant Stap...
EFFECTS OF SOLVENT POLARITY ON SOLVATION FREE ENERGY, DIPOLE MOMENT, POLARIZABILITY, HYPERPOLARIZABILITY AND MOLECULAR REACTIVITY OF ASPIRIN
Objective: The aim of the study is to explore the effects of solvent polarity on solvation free energy, dipole moment, polarizability, first order hyperpolarizability and different molecular properties like chemical hard...
AN IN SILICO STUDY OF NOVEL FLUOROQUINOLONES AS INHIBITORS OF DNA GYRASE OF STAPHYLOCOCCUS AUREUS
Objective: This study is an attempt to identifying an effective fluoroquinolones (FQ) s against STAPHYLOCOCCUS AUREUS (S. aureus) by in silico analysis of 150 (FQ) compounds using iGemDock v2.1 tool.Methods: Structure of...