ENHANCED APOPTOSIS IN MCF-7 HUMAN BREAST CANCER CELLS BY BIOGENIC GOLD NANOPARTICLES SYNTHESIZED FROM ARGEMONE MEXICANA LEAF EXTRACT
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To assess the in vitro anticancer activity of A. mexicana mediated gold nanoparticles on MCF-7 breast cancer cells.Methods: The present study reveals the synthesis and characterization of gold nanoparticles from aqueous extract of Argemone mexicana L. by biological method. UV-Vis spectroscopy, XRD, FT-IR, SEM and TEM analyses revealed that green synthesized gold nanoparticles were 26 ± 5 nm in size and spherical in shape. MCF- 7 Breast cancer cells were treated with different concentrations of green synthesised gold nanoparticles at different time intervals. Cytotoxicity activity and the mechanism of apoptosis were determined by morphological and chromatin cleavage assays.Results: Green synthesised gold nanoparticles showed dose-dependent cytotoxic and apoptotic effect in MCF-7 breast cancer cells at concentration of 100 μg/mL.Conclusion: The present investigation showed that Argemone mediated gold nanoparticles may be an alternative chemotherapeutic agent for treatment of breast cancer.Â
Authors and Affiliations
Selvaraj Varun, Sudha Sellappa
Keywords
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