Enzyme-linked immunosorbent assay in the diagnosis of hepatitis E in pigs
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. Hepatitis E has long been seen as an infectious disease that is detected in developing countries, both among humans and animals. Some strains infect only humans or animals, while others can infect both. Detection of anti-HEV in the blood is considered an indicator of infection with the virus. The prevalence of these antibodies may be an epidemiological marker of the incidence of HEV in the population. All these data, along with the presence of known species of animals that are seropositive for HEV significantly expanded the range of its carriers and its varieties. Strains of HEV in pigs, especially those detected in industrialized countries, are often associated with human cases, where any specific source of infection was not identified. The risk of virus transmission due to consumption of raw meat or exposure to pigs has already been proven in various countries. There is an assumption that transmission of HEV from pigs to humans is possible during slaughter and direct contact with pigs increases the risk of infection of workers. The goal of the work was to develop high-performance test kit for antibodies detection in the pig’s blood serum. Materials and methods. Surveyed and analyzed 1047 sera from pigs in farms and private sector of different regions of Belarus for the presence of anti-HEV-IgG using test systems CJSC “Vector-best” Vectored-E IgG, Novosibirsk, and also test kit of our own design. The ELISA development was carried out according to the conventional method. Results of research and discussion. Studies on the development of a test system for the detection of antibodies to HEV in pigs have shown that it is advisable to use as an antigen the gene-engineering polypeptides with antigenic sequence of the virus 1 and 3 genotype, conjugated with beta-galactosidase, for the sorption of polypeptides to polystyrene plates after pretreatment with ultraviolet radiation to increase the adhesion in sorption of recombinant antigen, in order to optimize the concentration of polypeptides. Sorption on polystyrene plates was 8 µg/ml in 0.005 M carbonate buffer pH-9.5, and breeding conjugate – rabbit antibody against IgG pigs TS30 antibodies is 1:10000. In the study of 1047 blood sera of pigs from farms and private farms 98.2% of samples contained specific antibodies to HEV human in different concentrations, which indicates the circulation of this virus in pig population in the Republic of Belarus. Сonclusions and prospects for further research. In the study of 1,047 sera of pigs from different farms, 98.2% of pigs’ sera contained specific antibodies to the human HEV in various concentrations, which indicates that it circulates among the pigs in Republic of Belarus.
Authors and Affiliations
Peter Krasochko, Alexander Kurdeko, P. P. Krasochko, Sergey Zhavoronok, Anastasia Arabey, Dmitry Borisovets, Tetyana Prokopenkova, Serghiy Nychyk
Keywords
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- EP ID EP405679
- DOI 10.31073/vet_biotech32(2)-34
- Views 132
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