Errors in the studies of enzymatic reactions resulting from unforeseen oxidation-reduction reactions
Journal Title: CHEMIK nauka-technika-rynek - Year 2014, Vol 68, Issue 4
Abstract
Enzymatic reactions are usually monitored using spectrophotometric measurements at one wavelength. Such measurements usually pose no risk, if a standard substrate that gives known product is used. However, if a mixture of compounds is studied, e.g. a substrate and potential inhibitors, this may lead to serious errors. In such cases unexpected reactions often occur. The potential inhibitors might be themselves enzyme substrates or might react with the product or intermediates of substrate conversion. Therefore, the expected product might not be produced or by-products are formed with spectra completely differently from the expected product or overlapping with this spectrum what might give totally false results.
Authors and Affiliations
B. Gąsowska-Bajger, H. Wojtasek
Keywords
Related Articles
- EP ID EP88376
- DOI -
- Views 95
- Downloads 0
Review of technology for cleaning biogas to natural gas quality
The article presents review of available biogas upgrading technologies to natural gas quality, as well as Polish achievements in this field. Article also took into account the national requirements relating to quality of...
Progress in work on the draft Industrial Emissions Directive
The paper presents the status of proceedings concerning the Directive of the European Parliament and of the Council on industrial emissions. In view of the slow progress in the implementation of the IPPC Directive, the E...
Charakterystyka i przyrodnicza użyteczność komunalnych osadów ściekowych w województwie dolnośląskim
W artykule podsumowano rzeczywiste wyniki badań osadów ściekowych, uzyskane w latach 1998–2012, z ponad 20. oczyszczalni południowo-zachodniej Polski – co daje pogląd o realnej sytuacji w tym sektorze gospodarki odpad...
Sustainable development – a threatened future
The concept of the eco-development came into being at 1st United Nations Conference on “Environment and development” in 1972, but this concept was formulated and defined for the first time in 1987 in the Brundtland repor...
REACH – chemikalia bezpieczne dla zdrowia i środowiska
Przez długie lata przemysł, także sektor chemiczny, był nastawiony głównie na poszukiwanie nowych materiałów i ulepszanie metod wytwarzania. Dopiero niedawno zaczęto zwracać uwagę na konieczność ograniczania negatywny...