EVALUATING THE STAINING POTENTIAL OF DYE EXTRACTED FROM BAPHIA NITIDA (CAMWOOD) ON MALARIA PARASITE
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 12
Abstract
This study examines the staining potential of aqueous and ethanolic extracts from Baphia nitida (indigenous herbaceous plant species) as a staining agent for malaria parasite. A total of 100 subjects were recruited for this study. 25 were apparently healthy subjects (controls) that had their blood films stained with the plant extract and the remaining 75 were subjects already diagnosed of malaria whose blood films were stained with the plant extract and Giemsa/Leishman stain (controls) respectively. The result obtained showed that dye extracted from Baphia nitida was unable to stain the malaria parasite. However, it was able to detect some of the blood cells such as red blood cells and white blood cells. It was noted that distilled aqueous hot extract with mordant gave the best staining result with 32% of the blood films stained with the above extract having an excellent staining quality, 16% being very good and 52% with a good staining quality. The distilled cold water extract (poor=60% and good=40%) stained much better than the ethanolic extract (poor=44%, good=52% and very good=4%). Generally, dye extract from Baphia nitida gave a less contrasted appearance in comparison to the conventional stains. The relationship between the method of dye extraction used and the quality of stain obtained was extremely significant (p<0.05). This implies that the quality of stain obtained following staining is largely dependent on the method of dye extraction use.
Authors and Affiliations
Madukwe David Uche Promise
Keywords
Related Articles
- EP ID EP634698
- DOI -
- Views 166
- Downloads 0
EVALUATION OF RESPONSE OF IMMUNE PARAMETERS IN HIV SUBJECTS ON ANTIRETROVIRAL THERAPY IN PORT HARCOURT
This cross sectional study was aimed at evaluating the response of immune parameters in HIV positive subjects on antiretroviral therapy in Port Harcourt, Rivers State, Nigeria. A total of three hundred subjects were invo...
ALOPECIA IN SHEEP DUE TO NUTRITIONAL DEFICIENCY IN SOUTH SINAI
This study was carried out on fourteen (14) sheep were housed in Raas Seder station in south Sinia. Among them, seven diseased sheep suffered from different degrees of alopecia, emaciation, lameness, and unthriftiness, a...
BREAST SELF-EXAMINATION PRACTICE AND ASSOCIATED FACTORS AMONG FEMALE HEALTHCARE PROFESSIONALS AT DIRE DAWA ADMINISTRATION, EASTERN ETHIOPIA
Background: Breast cancer is the most frequently diagnosed cancer in women worldwide. An estimated 521,900 breast cancer deaths occurred in women in 2012. Breast Self-Examination (BSE) is the recommended method for scree...
PREECLAMPSIA AND PREGNANCY INDUCED HYPERTENSION - CLINICAL AND LABORATORY EXAMINATIONS
Introduction: Pregnancy is a physiological condition in which regular controls are inevitable. Sometimes pathophysiological mechanisms develop such as preeclampsia and hypertension that can lead to further complication t...
PREVALENCE OF PROINSULIN LEVEL AMONG CONTROLLED AND UNCONTROLLED TYPE 2 DIABETIC SUDANESE PATIENTS
Background: Proinsulin concentrations are highly related to atherosclerosis and cardiovascular diseases in type 2 diabetes mellitus (type 2 DM). This study was carried out to assess serum level of proinsulin among type...