EVALUATION OF ANTIBACTERIAL, ANTIFUNGAL, ENZYME INHIBITION AND HEMOLYTIC ACTIVITIES OF CARYOPTERIS ODORATA FRACTIONS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 12
Abstract
The purpose of this study was to explore the antibacterial, antifungal, enzyme inhibition and hemolytic activities of various fractions of Caryopteris odorata (Ham. Ex Roxb.). The methanolic extract of plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially. Antibacterial activity was checked against Staphylococcus aureus, Bacillus subtilis, Pasturella multocida and Escherichia coli by the disc diffusion method using streptomycin sulphate, a standard antibiotic, as positive control. Polar fractions showed good antibacterial potential against these strains. These fractions also showed good MIC values. Antifungal activity was studied against four fungi i.e. Aspergillus niger, Aspergillus flavus, Ganoderma lucidum and Alternaria alternata by the disc diffusion method using fluconazole, a standard antifungal drug, as positive control. Ethyl acetate fraction was found active against A. niger and G. lucidum while chloroform and n-butanol fractions were active against A. flavus. Enzyme inhibition studies were performed against four enzymes i.e. α-glucosidase, butyrylcholinesterase, acetylcholinesterase and lipoxygenase. Ethyl acetate and n-butanol soluble fraction exhibited good activity against α-glucosidase with IC50 values 79.77 ± 0.97 and 82.77 ± 0.88 respectively. Chloroform soluble fraction showed moderate activity against butyrylcholinesterase (IC50 69.71 ± 0.95) while chloroform and ethyl acetate soluble fraction exhibited good activity against the lipoxygenase with IC50 values 65.28 ± 0.57 and 78.71 ± 0.69 respectively. Ethyl acetate fraction showed high toxicity i.e. 85.14 ± 0.83 %.
Authors and Affiliations
Muhammad Abbasi, Aziz-ur Rehman, Viqar Ahmad, Tauheeda Riaz, Farah Khlaid
Keywords
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