EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, ANTIHEMOLYTIC, AND PHYOCHEMICAL PROPERTIES OF FICUS BENJAMINA, FICUS INFECTORIA, AND FICUS KRISHNAE
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 3
Abstract
Objective: The study was conducted for the evaluation of antioxidant, antibacterial, antihemolytic, and phytochemical activity of Ficus benjamina (FB), Ficus infectoria (FI), and Ficus krishnae (FK). Methods: The antioxidant analysis of FB, FI, and FK was done by 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and nitric oxide (NO) scavenging assay. Evaluation was performed for antibacterial activity against Clostridium perfringens and Staphylococcus aureus by an agar well diffusion method. Hemolytic assay was performed with red blood cell suspension for evaluating antihemolytic activity. Standard protocols were used for phytochemical activity. Results: In the results of DPPH antioxidant activity assay, it was found that DPPH inhibition was significantly increased with increasing amount of extract. For NO scavenging assay, ANOVA revealed that the inhibition of NO is not significantly affected with an increase in the amount of extract used. Results of antibacterial activity revealed that methanolic extract of FI shows a maximum zone of inhibition (30.5 mm) against S. aureus and aqueous extract of FK shows a maximum zone of inhibition (28 mm) against C. perfringens. The antihemolytic activity of FB, FI, and FK was performed by measuring percentage inhibition of plant extracts at different concentrations. FK has shown maximum percentage inhibition activity, i.e., 28.64 % at 60 μg/ml, whereas FB shows minimum inhibition activity, i.e. 2.7 % at 40 μg/ml. Flavonoid content was found to be 0.593 μg/ml, 0.783 μg/ml, and 1.023 μg/ml, whereas phenolic content was found to be 0.267 μg/ml, 0.298 μg/ml, and 0.355 μg/ml for FB, FI, and FK, respectively. Conclusion: FB, FI, and FK extracts contain various phytochemicals which confirm that these plants can be used for therapeutic use and traditional medicine. The methanolic as well as aqueous extracts of the plants have shown the potential to kill the tested microorganism (C. perfringens and S. aureus) and hence can be used as an antibiotic and potential antibacterial.
Authors and Affiliations
PRABHJOT SINGH JASSAL, MONIKA SHARMA
Keywords
Related Articles
- EP ID EP603828
- DOI 10.22159/ajpcr.2019.v12i3.25351
- Views 108
- Downloads 0
AQBD ASSISTED SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF CEFIXIME TRIHYDRATE USING TWO OXIDATIVE COUPLING AGENTS
Objective: The present study focuses on developing two visible spectrophotometric methods for cefixime trihydrate (CFT) by the utilization of analytical quality by design space concept. The critical method variables were...
The THERAPEUTIC IMPORTANCE OF GUMS IN FOLK MEDICINE FROM EASTERN GHATS, ANDHRA PRADESH
Objective: The objective of the present study is to collect ethnobotanical information of gums of medicinal plants from Eastern Ghats of Andhra Pradesh. Methods: Intensive ethnobotanical field trips were conducted in th...
STUDY OF OUTWARD SIGNS, MICROSCOPY AND CHEMICAL COMPOSITION OF WALNUT
Objective: Walnut is a medicinal plant that is widely used in traditional medicine. We believe that the study of the medicinal plant material of walnut-fruit in the stage of milky-wax maturity and walnut bark is relevant...
IN SILICO DOCKING ANALYSIS OF YOHIMBINE ALKALOIDS FROM CATHARANTHUS ROSEUS AGAINST DENGUE FEVER
Objective: In this docking study was performed by the active site of NS2B-NS3 protease with selected plant alkaloids derived from Catharanthus roseus. On the basis of docking results, out of the nine alkaloids yohimbine...
ASSESSMENT OF PREVALENCE OF DEPRESSION IN CARDIAC PATIENTS AND ITS ASSOCIATION WITH THE USE OF BETA-BLOCKERS AND STATINS
Objective: The objective of the study is to assess the prevalence of depression among patients with cardiovascular disease and its association with the use of β-blockers and statins.Methods: This is a prospective observ...