EVALUATION OF ANXIOLYTIC POTENTIAL OF ETHANOLIC EXTRACT HYPERICUM HOOKERIANUM IN STRESS INDUCED SWISS ALBINO MICE
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 4
Abstract
The aim of the present work is evaluate to Anxiolytic profile of Hypericum hookerianum in stress induced Swiss albino mice. The study was carried out using Swiss albino mice (25- 30 g). The Anxiolytic effect of aerial parts of ethanolic extract of Hypericum hookerianum was evaluated by using behavioral analysis like Elevated plus maze (EPM) test, Open Field Test (OFT), Hole Board Test (HBT), Light dark exploration Test (LDE) in restraint stress induced animals. Behavioral test parameters for anxiety were assessed followed by biochemical parameters (lipid per oxidation, super oxide dismutase, catalase, glutathione per oxidase, reduced glutathione, etc.) and Diazepam 1 mg/kg served as a standard Anxiolytic drug, administered intraperitonealy. The results were shown that, ethanolic extract of H.hookerianum (Hh 100mg/kg and Hh 200 mg/kg, p.o.) significantly increased the percentage of time spent and number of entries in open arm in EPM. In LDE, the extract produced significant increase in time spent, number of crossing and decrease in the duration of immobility in light box. In OFT, the extract showed significant increase in number of rearings, assisted rearings and number of square crossed, all of which are demonstrations of exploratory behavior. Biochemical analyses revealed an increase in lipid per oxidation, depletion of super oxide dismutase, reduced glutathione, catalase activity and glutathione per oxidase in stress induced animals as compared to unstressed animal. Six days treatment of Hh (100 mg/kg, 200 mg/kg) comparable with Diazepam, significantly attenuated restraint stress-induced behavioral and oxidative damage. The results of the present study suggest that an ethanolic extract of H.hookerianum may possess Anxiolytic activity in stressed animals and provide a scientific evidence for its traditional claim.
Authors and Affiliations
S. Subakanmani , P. Umadevi
Keywords
Related Articles
- EP ID EP97909
- DOI -
- Views 83
- Downloads 0
MIDDLE EAST RESPIRATORY SYNDROME CoV: AN EVOLVING VIRAL THREAT TO HUMAN HEALTH
The Middle East respiratory syndrome (MERS) is a highly lethal respiratory disease. It is caused by a novel single-stranded, positive-sense RNA betacoronavirus (MERS-CoV). Specific drug treatment exists for MERS and infe...
DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND INDAPAMIDE IN TABLET DOSAGE FORM
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method based on Simultaneous equations method for simultaneous determination of amlodipine besylate and indap...
PHYTOCHEMICAL AND PHARMACOGNOSTICAL STUDIES OF MARTYNIA ANNUA PLANT
Martynia annua (Martyniaceae) is commonly known as “bichu”. In Ayurveda the leaves of the plant is useful in treatment of gargle, treat epilepsy, tuberculous, sore throat and also given local sedative effect. The whole...
REPRODUCTIVE SAFETY ASSESSMENT OF A POLYHERBAL ANTIHYPERTENSIVE MIXTURE IN GHANA
Medications designed to treat symptoms and cure chronic diseases can also cause unanticipated problems with the reproductive system; even in therapeutic doses. This study was therefore conducted on a commonly used polyhe...
ANTIBACTERIAL POTENTIAL OF CRUDE METHANOLIC EXTRACT OF Leonotis nepetifolia (L) R. Br
The methanolic extract of leaf, flower and stem of Leonotis nepetifolia was analyzed for antibacterial activity against eight pathogenic bacterial isolates viz., Escherichia coli, Pseudomonas aeruginosa, Shigella dysente...