Evaluation of CD40 and CD80 receptors in the colonic mucosal membrane of children with inflammatory bowel disease
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2015, Vol 22, Issue 4
Abstract
[b][/b][b]Introduction. [/b]The most prevalent inflammatory bowel diseases (IBD) include ulcerative colitis (UC) and Crohn’s disease (CD). Immune processes play a vital role in the etiopathogenesis of these conditions, involving both cellular and humoral response mechanisms. The aim of this study was to quantify CD40- and CD80-positive cells in the biopsy specimens of large intestinal mucosa from children with IBD. [b]Materials and method. [/b]The study comprised 38 children aged between 3–17 years (mean 11.5±3.7 years) – 20 boys (52.6 %) and 18 girls (47.4%). Eighteen patients were diagnosed with UC on the basis of clinical manifestation, endoscopic and histopathological findings. Mean age of this subgroup was 11.55±4.07 years. A group of 10 children (mean age 12.30±2.83) diagnosed with CD was also included. The control group comprised 10 IBD-free children (mean age 10.28±4.07 years). The surface expressions of CD40 and CD80 were analyzed in large intestine mucosa biopsy specimens, fixed in formaldehyde, embedded in paraffin, and cut with a microtome into 4 µm slices. [b]Results. [/b]The number of CD40- and CD80-positive cells in the large intestinal mucosa of children with Crohn’s disease and ulcerative colitis was significantly higher than in the controls. The highest number of CD40+ and CD80+ cells was observed in the caecal mucosal membrane of Crohn’s disease patients and in the rectal mucosa of individuals with ulcerative colitis. [b]Conclusion.[/b] IBD is characterized by elevated, segment-specific, expression of CD40 and CD80.
Authors and Affiliations
Barbara Kamińska, Izabela Roszko-Kirpsza, Piotr Landowski, Agnieszka Szlagatys- Sidorkiewicz, Katarzyna Guzińska-Ustymowicz, Elżbieta Maciorkowska
Keywords
Related Articles
- EP ID EP81037
- DOI -
- Views 150
- Downloads 0
Concentration of fungal biomass and trichothecenes in different parts of einkorn.
Analyses of ergosterol (ERG), adenosine-5?-triphosphate (ATP) and groups A and B trichothecenes were performed in three Triticum monococcum cultivars registered in Germany (Albini, Terzino and Tifi), grown in the organic...
Short-term effects of a randomized controlled worksite relaxation intervention in Greece
objective. To evaluate the short-term benefits of simple relaxation techniques in white-collar employees. materials and methods. The study was a two-arm parallel group randomized controlled trial. 152 employees were ra...
Managing water safety in healthcare. Part 1 – Strategies and approaches for waterborne pathogen control
Summaries in 2 parts are presented from a conference held in London at the Royal Society for Public Health on 16-17 May 2012, on the latest developments in dealing with waterborne hospital-acquired infections (nosocomial...
Geostatistics – a tool applied to the distribution of Legionella pneumophila in a hospital water system
[b]Introduction.[/b] Legionnaires’ disease is normally acquired by inhalation of legionellae from a contaminated environmental source. Water systems of large buildings, such as hospitals, are often contaminated with legi...
Strength training and diet supplementation in the Polish Army on the example of a selected unit
of this article was to determine strength training practices and the intake of supplements by Polish Army soldiers. Attitudes toward physical fitness and the level of dietary knowledge among the soldiers were also examin...