EVALUATION OF CELL SURFACE ANTIGEN EXPRESSIONS IN CASE OF CHRONIC LYMPHOCYTIC LEUKEMIA
Journal Title: Kocatepe Medical Journal - Year 2022, Vol 23, Issue 2
Abstract
OBJECTIVE: Immunophenotyping is critical for the diagnosis of B cell malignancy. In this study, we aimed to evaluate the cell surface antigen expression percentages and densities by flow cytometry method in patients diagnosed with Chronic Lymphocytic Leukemia (CLL) clinically and morphologically. MATERIAL AND METHODS: Flow cytometry results of peripheral venous blood of 33 CLL patients (12 females / 21 males) were retrospectively analyzed. First, lymphocytes were marked on the CD45-Side Scatter chart and antibody expression percentages and densities in this population were determined. RESULTS: It was observed that all patients had CD5, CD19, CD20, CD23, CD200 and CD43 antigen expressions as in the classical pattern. Unlike the classical pattern, the expression of FMC7, which is not expected is over 30% in eight patients. While 6 of the cases showed kappa monoclonality, 15 of them showed lambda monoclonality, it was seen that both light chain expressions were below 30% in 11 cases. ZAP 70 expression was found in three cases (16%, 23%, 42%). Looking at the average percentage of antigen expressions of all cases, it is seen that CD5, CD19, CD20, CD23, CD200 and CD43 are above 80%. CONCLUSIONS: Surface antigen expressions recommended to be checked in the diagnosis of CLL may not follow the rules defined in each case. In these cases, there is a need to search for more specific additional cell markers to enter the diagnostic algorithm.
Authors and Affiliations
Filiz YAVAŞOĞLU, Çiğdem ÖZDEMİR, Tülay KÖKEN
Keywords
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- EP ID EP702626
- DOI https://doi.org/10.18229/kocatepetip.832270
- Views 90
- Downloads 0
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