Evaluation of Dark Field Microscopy for the Early Diagnosis of Leptospirosis- A Prospective Study
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 8
Abstract
Background: Leptospirosis is a worldwide public health problem. Early diagnosis of leptospirosis is essential since antibiotic therapy is useful only when it is initiated early in the course of illness. The purpose of this study was to evaluate dark field microscopy for early diagnosis of leptospirosis. Materials and Methods: A total of 81 blood samples were collected from the suspected patients. Blood samples (5ml each) were collected aseptically in two sterile SV10 vials, one containing 500 µl 1% sodium oxalate solution PH 8.0 and another in dry tube. The former sample was used for dark field microscopy and the other was used for serodiagnosis by MAT and ELISA. Blood was also collected from 50 healthy blood donors and tested by DFM, ELISA and MAT. Results: It was found that found that DFM sensitivity is 55.55% (45/81), IgM ELISA is 65.43% (53/81) and MAT is 54.32% (44 /81). On evaluation of DFM we found, its sensitivity is 61.64% (45/73), specificity is 95.23% (40/42), positive predictive value is 95.74% (45/47) and negative predictive value is 58.82% (40/68). Conclusion: Sensitivity of DFM was found to be lesser than that of ELISA but slightly more than that of MAT. Evaluation of DFM showed good sensitivity and greater specificity. Hence DFM can be performed using a good dark field microscope and anticoagulant added blood for the early diagnosis of leptospirosis along with ELISA.
Authors and Affiliations
Binay Kumar Singh
Keywords
Related Articles
- EP ID EP518455
- DOI -
- Views 63
- Downloads 0
Dry Eye and Corneal Sensitivity in Patients with Type 2 Diabetes Mellitus
Background: Ocular surface disorders in diabetes mellitus have gained significant attention in recent years, as there is increased reporting of the association of dry eye and diminished corneal sensitivity in these patie...
Assess the Knowledge Regarding Intra Hospital Transport of Critically ill Patients among Nursing Staff of Dhiraj Hospital, Waghodia
“Assess the knowledge regarding intra hospital transport of critically ill patients among nursing staff of Dhiraj Hospital, Waghodia, at. Piparia, Vadodara. Objectives of the study To assess the knowledge regarding int...
Intracranial Plasmacytoma with Unilateral Vision Loss: An Unusual Initial Presentation of Multiple Myeloma
Plasma cell neoplasms are clonal proliferation of plasma cells that produce a single class of immunoglobulin. Among the plasma cell neoplasms, both solitary plasmacytoma of bone and extra-osseous plasmacytoma can progres...
Therapeutic Benefits of Garlic against Alloxan-Induced Diabetic in Rats
Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia of diabetes is associated with longterm da...
Prevalence of Intestinal Parasitic Infection in Children of Slum Area of Dhanbad, Jharkhand
The presence of intestinal parasitic infection in slum area children’s is indicative of poor educational background, Poor sanitation and unsafe drinking water increases the risk of parasitic infection.