EVALUATION OF HEPATOPROTECTIVE EFFECT OF CALOTROPIS PROCERA (AIT) R.BR. ROOT EXTRACT AGAINST PARACETOMOL INDUCED HEPATO-OXIDATIVE STRESS IN ALBINO RATS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 1
Abstract
In the present study, Calotropis procera (Asclepediaceae) was evaluated for its possible hepatoprotective and antioxidant potential. Hepatoprotective activity of the methanol extract (MCP) of the root was determined using paracetamol-induced liver injury in rat. The animals were weighed each and divided in groups of five. Liver damage was achieved by injecting Paracetamol (2 g/kg). The treatment groups pretreated with MCP Group-IV (200mg/kg) and Group-V (400mg/kg). Silymarin was used as reference standard drug. At the end of 7 days, blood was collected, liver extracted, weighed, processed for histopathological assessments and for antioxidant activity. Alteration in the levels of biochemical markers of hepatic damage like serum transaminases(AST,ALT), alkaline phosphatase (ALP), bilirubin, cholesterol, high density lipoprotein (HDL) and tissue GSH were tested in all the groups. The MCP extract exhibited a significant hepato-protective effect by lowering the elevated serum levels of AST, ALT, ALP, total and direct serum bilirubin, cholesterol and significantly (p<0.05) increased HDL and moderately increased total protein and albumin in a dose dependent manner These biochemical observations were supplemented by histopathological examination of liver sections. Further, the effects of the active fractions on antioxidant enzymes also have been investigated to elucidate the possible mechanism of its hepatoprotective activity. The MCP extract exhibited a significant effect (P<0.05) in dose dependent manner by modifying the levels of reduced glutathione, superoxide dismutase, catalase activity and malondialdehyde equivalent, an index of lipid peroxidation of the liver. These findings suggest the use of this plant for the treatment of liver toxicity in oriental traditional medicine
Authors and Affiliations
Prakash Patil , K Prasad , M Nitin , M Vijay Kumar, K Sreenivasa rao
Keywords
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