EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 5
Abstract
Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.
Authors and Affiliations
Iswariya S. , Uma T. S.
Keywords
Related Articles
- EP ID EP575630
- DOI 10.22159/ijpps.2017v9i5.16708
- Views 69
- Downloads 0
UTILIZATION OF ALCOHOLIC BEVERAGE DISTILLERAY WASTE WATER FOR PRODUCTION OF DOCOSAHEXAENOIC ACID
Objective: The marine thaustochytrid Schizochytrium sp known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications. In the present study, distillery waste was studi...
EXPLORATION OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF MOMORDICA CYMBALARIA FENZL LEAVES EXTRACTSEXPLORATION OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF MOMORDICA CYMBALARIA FENZL LEAVES EXTRACTS
Objective: The target of this current research work sought to analyze the antioxidant and antimicrobial activities of ethyl acetate and hydro-alcoholic extracts of Momordica cymbalaria leaves through various in vitro ass...
PHARMACOGNOSTIC STUDY OF CALLISTEMON CITRINUS L. BARK
Objective: Pharmacognostic study of Callistemon citrinus L. barkMethods: The bark of Callistemon citrinus L. was shade dried and powdered mechanically. The powdered material was used for further Pharmacognostic study. Th...
DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING UPLC METHOD FOR THE ESTIMATION OF R-TELAPREVIR IN DRUG SUBSTANCE AND PHARMACEUTICAL DOSAGE FORM OF TELAPREVIR BY ENHANCED APPROACH
Objective: To develop a novel, simple, precise and stability indicating reverse phase ultra-performance liquid chromatographic (RP-UPLC) method and validate as per ICH guidelines for the quantification of R-Telaprevir in...
ENCAPSULATION OF RED GINGER OLEORESIN (ZINGIBER OFFICINALE VAR RUBRUM) WITH CHITOSAN AS WALL MATERIAL
Objective: This research aims to determine the effect of the spray drying condition against encapsulation efficiency and characterization microcapsules of red ginger oleoresin.Methods: Preparation of encapsulation begun...