EVALUATION OF IN VITRO ANTIOXIDANT AND ANTI-PROLIFERATIVE EFFECT OF DEFATTED EXTRACT OF COCOS NUCIFERA KERNEL ON HUMAN BREAST CANCER CELL LINE
Journal Title: International Journal of Pharmacognosy - Year 2018, Vol 5, Issue 3
Abstract
The present study was designed to evaluate the in vitro antioxidant effects and potential benefits of using coconut kernel extract in preventing the proliferation of human breast cancer cell line, MCF-7. Defatted extract from coconut kernel (CKf) was evaluated for its radical scavenging, prevention of human LDL oxidation, cytotoxic, apoptotic and nuclear effects on MCF-7 cells. Intracellular production of reactive oxygen species (ROS) and superoxide radical were studied. It was showed that CKf significantly scavenged the free radicals and prevented LDL oxidation. MCF-7 cell viability was found to be decreased in a dose dependent manner following treatment with CKf. AO-EB and DAPI staining confirmed that treatment with CKf, induced nuclear damage and apoptosis as distinctly visualised in cells, Our observations suggest that nuclear/cellular damage and poor mitochondrial membrane potential (MMP) appears to be an indirect consequence of the excessive generation of reactive oxygen species (ROS) and superoxide radicals.
Authors and Affiliations
Kottayath Govindan Nevin et al.
Keywords
Related Articles
- EP ID EP431962
- DOI -
- Views 59
- Downloads 0
REGULATION OF WNT GENES IN STEM CELLS DEVELOPMENT AND ORGANOGENESIS
Stem cell biology, many signals regulated stem cells and organ development. Wnt factors play a crucial role in stem cells development and induced signaling pathway in stem cells. Wnt signals classified as a canonical and...
STUDY OF PLANTS WITH D-CHIRO-INOSITOL AND ITS DERIVATIVES ON DIABETES
Diabetes is an endocrine disorder and it is due to deficiency in secretion or action of insulin. The increased prevalence of diabetes in recent decades has made it to serious risk for public health. Human always has grea...
ANTIDIARRHEAL, ANTIDIABETIC, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACTS OF LEAVES OF CLERODENDRUM VISCOSUM (VENT.)
The present study was aimed to investigate the possible antidiarrheal, antidiabetic, antioxidant and antimicrobial activity of methanolic extracts of leaves of Clerodendrum viscosum (Vent.) Different screening models are...
MUSA PARADISIACA – A WONDER FRUIT
Over the past centuries phytochemical and phyto-pharmacological sciences established the compositions, biological activities and health promoting benefits of numerous botanical products. Musa paradisiacal also known as M...
ANATOMICAL STUDIES ON LEAF OF CADABA FRUTICOSA (L.) DRUCE
Cadaba fruticosa (L.) Druce belongs to the family Capparaceae. It is woody, erect, glandular- pubescent shrubs. It is used in the folk system of medicine. Anti-rheumatic, anthelmintic and antibacterial properties it is a...