Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia
Journal Title: International Journal of Reproductive BioMedicine - Year 2017, Vol 15, Issue 5
Abstract
Background: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology. Objective: To compare the level of intracellular anion superoxide (O2–), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men. Materials and Methods: In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40% ) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2– and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test. Results: The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2– levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2– and HSPA2 in the case group (r=0.041, p=0.79). Conclusion: We suggest that the increase in intracellular O2–, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients.
Authors and Affiliations
Parvin Sabeti, Fardin Amidi, Seyed Mahdi Kalantar, Mohammad Ali Sedighi Gilani, Soheila Pourmasumi, Atefeh Najafi, Ali Reza Talebi
Keywords
Related Articles
- EP ID EP183834
- DOI -
- Views 139
- Downloads 0
Twin pregnancy in the unicornuate uterus and non-communicating rudimentary horn: A case report
Background: A unicornuate uterus is present in 0.1% of the general population. This müllerian anomaly carries significant obstetrical risk including abortion, preterm delivery, and rudimentary horn ruptures. Case: The p...
Y chromosome microdeletions frequency in idiopathic azoospermia, oligoasthenozoospermia, and oligospermia
Background: Genetic factors are candidates for about 30% of male infertility with sperm production-related abnormalities. Y chromosome microdeletions are responsible for around 10% of male infertility. These microdeletio...
Effects of autologous platelet-rich plasma on implantation and pregnancy in repeated implantation failure: A pilot study
Background: Repeated implantation failure (RIF) is a major challenge in reproductive medicine and despite several methods that have been described for management, there is little consensus on the most effective one. Obje...
The stem cell session of the 7th Yazd International Congress and Student Award in Reproductive Medicine held at Shahid Sadoughi University of Medical Sciences
This paper summarizes the proceedings of the stem cell session of the “7th Yazd International Congress and Student Award in Reproductive Medicine” held at Shahid Sadoughi University of Medical Sciences, Yazd, Iran, on 28...
The role of autoimmunity in premature ovarian failure
Premature ovarian failure (POF) is a heterogeneous syndrome with several causative factors. Autoimmune mechanisms are involved in pathogenesis of 4-30 % of POF cases. The present review focuses on the role of autoimmunit...