Evaluation of lipase activity in some Aspergillus species
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 8
Abstract
Lipase is an enzyme which is able to hydrolyze the esters and breakdown the triglycerides into glycerol and fatty acids. This enzyme is so noteworthy in organic chemistry and industry. Fungi are one of the microorganisms producing this enzyme. In this paper, screening was done between 10 species of fungi, Aspergillus, to determine lipase activity. A specific medium containing minerals and soybean oil was used in order to induce the lipase production. Three days after inoculation, amount of the enzyme was evaluated every other day for 20 days. Evaluation was done using colorimetric method with substrate para-nitrophenyl palmitate in wavelength 410 nm by a spectrophotometer. According to the results, all isolates were able to produce the lipase and degrade the triglyceride; but the most lipase activities were related to Aspergillus caespitosus and A. Ostianus 39.9 U/ml and 36.01 U/ml respectively. The most biomass production was related to A. caespitosus. These native isolates with high lipase production potential can be new candidates to industrially produce lipase.
Authors and Affiliations
MA Tajick, S Leylaei, T elmadarrehei
Keywords
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