Evaluation of Phytochemical compounds by TLC and FT-IR analysis of Cipadessa baccifera (Roth) Miq. and Elytraria acaulis (L.f) Lindau
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
The present study was carried out to investigate the medicinal bioactive substances present in the petroleum ether, toluene and methanolic extracts Cipadessa baccifera and petroleum ether and methanolic leaf extracts of Elytraria acaulis. In the present investigation, chromatographic techniques such as Thin Layer Chromatography (TLC) analysis was used to separate the bioactive compound from the crude leaf extracts of Cipadessa baccifera and Elytraria acaulis. The solvent system of TLC was Methanol: Chloroform (1:1, 6:4, 7:3 and 9:1) used and Rf value was recorded. TLC chromatogram was subjected to bioautography to check the antibacterial activity against Staphylococcus aureus. For FTIR analysis the leaf extract of Cipadessa baccifera and Elytraria acaulis was focused in the transmittance ranging from 400-4000cm-1 on a Perkin Elmer Spectrum and the characteristic peak values and their functional groups were detected. From TLC analysis spots were identified with Rf values 0.76 and 0.83 was compared with literature data showed that the presence of flavonoid compound as quercetin. Bioautography showed zone of clearance indicates the presence of active components inhibits the growth of Staphylococcus aureus when the chromatogram is sprayed with p–iodonitrotetrazolium violet (INT) spray
Authors and Affiliations
Jeevitha DS, Manjunath Kiragandur
Keywords
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