EVALUATION OF PUṄKAM PŪ CŪRAṆAM AND IDENTIFICATION OF PUṄKAM PŪ BY HPTLC METHOD
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2018, Vol 4, Issue 7
Abstract
Siddha medicines mainly comprises of plant parts, metals, minerals and some animal substances. Nowadays, the purity and quality of drugs were hampered by adulteration. So, it is mandatory to ensure the quality and safety of drugs. The aim of the present study is to standardize a Siddha formulation, Puṅkam pū cūraṇam and its herbal ingredient Pongamia pinnata (L.) Pierre (Puṅkam pū) by applying suitable standards and using modern techniques. The identification of the herbal ingredient in the cūraṇam is also performed with these methods. Puṅkam pū cūraṇam and Puṅkam pū were subjected to powder microscopical studies, physico-chemical analysis, preliminary phytochemical studies and HPTLC analysis. Physico-chemical parameters such as total ash, acid insoluble ash, solubility in alcohol and water and loss on drying at 105ºC of the cūraṇam and the ingredient were determined by standard methods. Preliminary phytochemical studies revealed the presence of alkaloids, flavonoids, steroids, tannins, carbohydrates, phenols and terpenoids in the cūraṇam and in the ingredient. Organoleptic characters (colour, odour and taste) of the cūraṇam were noted. TLC chromatogram & HPTLC finger print profiles were documented at 254 nm and 575 nm after derivatisation. The present study ensures the quality of the Puṅkam pū cūraṇam and its ingredient Puṅkam pū by evaluating the powder characteristics, physico-chemical constants and HPTLC fingerprints.
Authors and Affiliations
Anitha John
Keywords
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