Evaluation of Ready-to-eat Polyethylene Packed Pawpaw (Carica papaya) for the Presence of Antibiotic Resistant Escherichia species
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 12, Issue 4
Abstract
Aims: The study examined ready-to-eat cut polyethylene packed pawpaw (Carica papaya) sold in the University of Port Harcourt community for the presence of antibiotic resistant Escherichia species. Study Design: The samples were randomly purchased from vendors, who openly display them in trays and composite analysed in duplicate. Place and Duration of Study: Department of Microbiology, University of Port Harcourt between January and June 2015. Methodology: A total of 50 ready-to-eat polyethylene packed pawpaw were examined for the presence of presumptive E. coli 0157:H7 and non-0157:H7 E. coli, using MacConkey-Sorbitol agar and eosin methylene blue agar. Isolated were confirmed on the basis of cultural morphology, physiology and biochemical characteristics. Screening for antibiotic susceptibility was done using the disk diffusion method involving Mueller Hinton agar. Results: Of the 50 samples examined, 34 (68%) were positive for Escherichia species, with counts ranging from 1.6 to 3.5 ×104 cfu/g. Presumptive 0157:H7 E. coli accounted for 5.88% while non-0157:H7 E. coli accounted for 94.12%. The resulting isolates showed varying resistance to augumentin (5.88%), amoxicillin (97.06%), cloxacillin (73.53%), cotrimoxazole (95.58%), erythromycin (5.88%), gentamicin (14.71%), nitrofurantoin (85.29%) and tetracycline (58.82%). Conclusion: The study clearly reveals that consumption of ready-to-eat cut pawpaw from vendors can be a potential risk for food borne outbreaks because of their contamination level by E. coli and the variable resistance patterns in response to different antibiotics used in the study.
Authors and Affiliations
O. C. Eruteya, A. Nwayanwu
Keywords
Related Articles
- EP ID EP318117
- DOI 10.9734/JABB/2017/32352
- Views 89
- Downloads 0
Evaluation of Latent Infection, Bio-deterioration and Yield of Colocasia esculenta (L.) Schott, Caused by Taro Leaf Blight Disease (Phytophthora colocasiae Raciborski)
Taro leaf blight disease caused by Phytophtora colocasiae is a potential economic disease of the taro crop growing regions of Cameroon and other humid tropical regions of West and Central Africa. Studies were conducted a...
Computational Analysis of Genomic Regions of Human Insulin Receptor Gene
Insulin plays an essential role in metabolic control of multiple cellular processes. A number of DNA sequences that regulate transcription of the insulin gene have already been identified. The regulation of carbohydrates...
Production of Monoclonal Antibodies in Transgenic Plants
Antibodies are one of specialized immune protein and innovative therapeutic agents that provides effective alternatives to treating various human diseases. The existing fermenter based process of production of great valu...
Delimitation of the Pore in Tweety Homolog 1 Channels: A Model-guided Approach
Tweety homolog proteins have been indicated as ´putative anion channels´, but yet there is some lack of definitive data in this regard. Here, we focused on the human Tweety homolog 1 protein (hTTYH1), and elaborated a mo...
Variability of Phenolic Compounds and Biological Activities among Wormwood Extracts Originated from Different Bioclimatic Zones
Aim: Wormwood is an aromatic spice widely used as flavor, antioxidant and antimicrobial agents. In this study the variability of total phenolics, total flavonoids, antioxidant, antibacterial and antifungal activities amo...