Evaluation of the proliferative activity of aqueous extracts from three medicinal plants in murine spleen cells
Journal Title: European Journal of Biological Research - Year 2016, Vol 6, Issue 3
Abstract
There are number of medicinal plant products (leaves, root, stem bark, seeds etc.) including its metabolites i.e. primary (protein) and secondary (flavonoids, terpenoids, glycosides etc.) that have been used as immunomodulatory (stimulatory, suppressive or adjuvant) agents. For the last several years, researchers focused on various immunopharmacological aspects especially its therapeutic action of these medicinal plants against various diseases. The objective of our study was to identify and evaluate the action of aqueous leaves extract from these medicinal plants i.e. Azadirachta indica, Syzygium cumini and Prosopis spicigera on the development of spleen cells (ex vivo cellular proliferation assay) from mice using hepatitis B vaccine containing surface antigen (HBsAg; 20 µg/ml) and also estimated its pro-inflammatory (IFN-gamma and TNF alpha) cytokines from spleen cell culture supernatant. For these studies, mice were immunized subcutaneously with HBsAg on day 0. After three days, spleens were excised aseptically and prepared single cell suspension (106 cells/ml) using variable doses of aqueous leaves extract (0.625-5 mg; 50 µl) incubated with or without HBsAg. After 48 h, cell culture supernatant was collected for the estimation of Th1 (IFN-gamma and TNF alpha) cytokines. However, cell proliferation was revealed by blue tetrazolium reduction assay (MTT) and cytokines by ELISA. The results showed these aqueous leaves extracts showed dose-dependent reduction of cell proliferation including pro-inflammatory cytokines. Overall, these medicinal plants showed immunosuppressive activity in ex vivo murine model based studies.
Authors and Affiliations
Amit Gupta, Sushama R. Chaphalkar
Keywords
Related Articles
- EP ID EP230360
- DOI 10.5281/zenodo.57997
- Views 143
- Downloads 0
Toxicological investigation and anti-reproductive effect of phyto-molluscicide against harmful aquatic snail
In this study attempt was made by using active component Taraxerol isolated from medicinal plant of India, Codiaeum variegatum (Family: Euphorbiaceae) to control harmful disease schistosomiasis in domestic animals caused...
Nutritive values of some edible forest tree seeds in Makurdi-Benue, Nigeria
The seeds of Adansonia digitata, Afzelia africana, Moringa oleifera, Prosopis africana and Terminalia catappa in Makurdi, Nigeria were analyzed in water-soluble forms to determine their minerals (calcium, phosphorus, pot...
Aspergillus terreus occurrence in middle of Upper Egypt, genetic variation and production of itaconic and mevinolinic acids
Several isolates of Aspergillus terreus were identified and isolated as fungal flora from sixty-four out of seventy screened sources in middle of Upper Egypt. A. terreus was common in plant roots (dill, geranium and petu...
Silver tolerance and silver nanoparticle biosynthesis by Neoscytalidium novaehollandae and Trichoderma inhamatum
Development of reliable and eco-friendly processes for synthesis of metallic nanoparticles is an important step in the field the applications of nanotechnology. Biological systems provide a useful option to achieve this...
Allometric models for branch biomass production: assessment of rapid growth trees for bio-energy in Northern Iran
The increase of atmosphere carbon dioxide (CO2) as a result of burning fossil fuel is becoming a global issue. Biomass fuels are an environmental friendly alternative for energy supply. Among the sources of biomass, fore...