Evaluation of Time to Detection of Bacteria and Yeasts Isolated From Blood with BACTEC 9120 Automated Culture System
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Introduction: Conventional diagnostic methods for culture of normally sterile body fluids involve their inoculation directly into agar media or into various blood culture media like Brain Heart Infusion (BHI) broth. The yield of these methods is limited due to small volume of fluid cultured, overgrowth by contaminated microflora and phagocytosis of microorganisms by white blood cells & other contributing factors. BACTEC automated culture system for microbial isolation has many advantages over the conventional culture methods such as reduced time to detection, isolation of fastidious microorganisms, simpler transportation of specimen to laboratory and microbial growth positivity even after initiation of antimicrobial therapy. Aims and objectives: To isolate and identify the bacterial pathogens from blood & to compare the time needed for the detection of microorganisms by BACTEC 9120 microbial detection system. Materials and Methods: The study was carried out in the Department of Microbiology, Dr. Rajendra Prasad Government Medical College (DRPGMC) & Hospital, Kangra at Tanda over a period of six months w.e.f. October, 2014 to March, 2015. Blood suspected to be infected was included in the study. Statistical analysis was done using standard statistical techniques BACTEC system consists of self-contained incubator, agitator and detection device with capacity of 120 bottles in BACTEC 9120 model. The sample to be tested is inoculated into the vial which is entered into the BACTEC instrument for incubation and periodic reading. Each vial contains a sensor which responds to the concentration of CO2 produced by the metabolism of microorganisms or the consumption of oxygen needed for the growth of microorganisms. The sensor is monitored by the instrument every ten minutes for an increase in its fluorescence, which is proportional to the increasing amount of CO2 or the decreasing amount of O2 present in the vial. A positive reading indicates the presumptive presence of viable microorganisms. BACTEC Blood culture bottles contain soya bean casein broth & SPS (Sodium polyanethol sulfonate). Results and Conclusions: BACTEC automated culture system for microbial isolation has many advantages such as reduced time to detection, isolation of fastidious microorganisms, simpler transportation of specimen to laboratory and microbial growth positivity even after initiation of antimicrobial therapy. The presence of resins and lytic agents like saponin in BACTEC Peds Plus /F may decrease the inhibitory effects of antibiotics and favours the release of phagocytosed organisms. Also the dilution effect of inoculum into liquid medium in BACTEC culture bottle may decrease the inhibitory effect of inhibitors present in the fluids.
Authors and Affiliations
Ravi Kumar, Anuradha Sood, Kamlesh Thakur, Samriti Chauhan
Keywords
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- EP ID EP546848
- DOI 10.21276/ijmrp.2017.3.4.037
- Views 87
- Downloads 0
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