Evalution of “indirect fluorescent antibody test as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2016, Vol 4, Issue 0
Abstract
In the present study, Indirect Fluorescent antibody test (iFAT) has been used as the “screening test” for the detection of MAP bacilli in the milk samples of lactating domestic livestock. A total of 372 milk samples from lactating animals were screened by iFAT and results were compared with microscopy of milk samples and milk ELISA test. Comparative analysis of the results of three tests showed that iFAT had fairly good sensitivity (84.7%) and specificity (90.4%), with respect to ZN staining (microscopy) with kappa value of 0.735 and ‘good’ strength of agreement. Similar comparison with milk ELISA test revealed, sensitivity (73.3%) and specificity of (72.6%) with kappa value of 0.443 and strength of agreement was moderate. Lower or higher difference in sensitivity and specificity of iFAT with respect to ZN staining and milk ELISA may be due to the difference in detection ‘target’ of the test i.e., antigen or antibody. It was concluded that iFAT was a reliable and sensitive diagnostic test for the detection of MAP in milk and can also be used for the ‘mass screening’ of the milk samples.
Authors and Affiliations
Shoor Vir Singh, Bjorn John Stephen, Manju Singh, Saurabh Gupta, Kundan Kumar Chaubey, Sahzad . , Sujata Jayaraman, Tarun Kumar Sachan, Gajendra Kumar Aseri, Manali Dutta, Jagdip Singh Sohal, Kuldeep Dhama, Shivalingappa Yamanappa Mukartal, Doddamane Rathnamma
Keywords
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- EP ID EP429693
- DOI 10.18006/2016.4(5).533.540
- Views 59
- Downloads 0
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