EX VITRO ACCLIMATIZATION AND CONSERVATION OF THE PLANT MATERIAL OBTAINED IN VITRO AT SOME FOREIGN AND AUTOCHTHONOUS VARIETIES OF SOLANUM TUBERSOSUM L
Journal Title: Natural Resources and Sustainable Development - Year 2013, Vol 3, Issue 1
Abstract
In vitro acclimatization and conservation of the plant material obtained in vitro is an important stage for the units of micro-propagation and production of the potato tubers this way. The capacity of acclimatization of the foreign variety of Desirée potato and of the autochthonous variety Super potato was followed, after a period of in vitro culture of about two months on mediums with or without phytohormones, stimulating or not the differentiation of mini-tubers. Acclimatization refered, on one hand, to the mini-tubers obtained in vitro, and on the other to the neoplantlets which did not differentiate tubers. The material obtained in vitro acclimatized ex vitro after about 3 months, the capacity of acclimatization depending on the completion of the stages of acclimatization, on the variety or on the potato population, on the preliminary preparation stage still in the in vitro conditions, through an additional light treatment, on the culture substratum and on the conditions of its sterilization against pathogens agents with antifungal treatment, also applied for ensuring a good nutrition, the size of the tubers (the diameter of the tuber) etc. Desirée variety acclimatized in a percentage of 80%, and the autochthonous one, Super, in a percentage of 95%, depending on the type of acclimatized material (tubers or rooted neoplantlets). After completing these stages the obtained material (tubers) corresponds to merchandising and to the internal and external exchange, being accompanied by a phytosanitary certification issued by the law of the country which produced the plant material.
Authors and Affiliations
Eliza Maria Agud, Maria Zăpârțan, Vasile Laslo
Keywords
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