EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
Journal Title: Сільськогосподарська мікробіологія - Year 2015, Vol 21, Issue
Abstract
It was established that B. mucilaginosus С-3 requires introduction of nitrogen source into the culture medium in form of nitrate as the most optimal for maximal accumulationof bacterial biomass and sporulation process. Introduction ammonium form was less efficient. It was shown thet the intensity of nitrogen source use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum the optimal concentration of potassium nitrate was 2,5 g/l resulting in 10*10 cells/ml, while under the use of vegetative cells the optimal concentration of potassium nitrate was 1,0 g/l leading to the accumulation of 10*9 cells/ml. Use of spore inoculum had ensured the maximum productivity of extracellular polysaccharide synthesis. With the higher number of B. mucilaginosus subcultures in a vegetative state the efficiency of the exopolysaccharide synthesis decreased: after the second passage - by 20,0%, after the fourth - by 56,5%, after the sixth - at 127,8%. After eight passage the culture loses its ability to synthesize polysaccharide, especially on the medium with the N/C ratio 1:3 and resume this ability only after sporulation and the significant increase of the N/C ratio.
Authors and Affiliations
I Malynovska
Keywords
Related Articles
- EP ID EP520080
- DOI -
- Views 99
- Downloads 0
EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
It was established that B. mucilaginosus С-3 requires introduction of nitrogen source into the culture medium in form of nitrate as the most optimal for maximal accumulationof bacterial biomass and sporulation process....
FUNCTIONING OF NITROGEN FIXATION SYMBIOSIS AND PEAS PRODUCTIVITY UNDER THE APPLICATION OF DIFFERENT TYPES AND DOSES OF FERTILIZERS
The efficiency of different types and doses of fertilizers and pre-sowing bacterization of pea seeds (Starter variety) on the formation and functioning of symbiotic nitrogen fixation system, crop productivity and protein...
TRENDS IN BIOLOGICAL PROCESSES IN LEACHED CHERNOZEM UNDER CULTIVATION OF SPRING BARLEY WITH VARIOUS SPECIES AND RATES OF FERTILIZERS
The influence of the aftereffects of organic fertilizers and direct action of minerals on the development of microorganisms of nitrogen and carbon cycles, as well as the course of individual biological processes has been...
THE IMPACT OF BIOLOGICAL PREPARATIONS ON THE EFFECTIVE SOIL FERTILITY AND MINERAL NUTRITION OF APRICOT AND CHERRY PLUM SEEDLINGS
The effect of microbial preparations (MP) use, created on the basis of nitrogen-fixing and phosphorous mobilising bacteria as well as complex of microbial preparations (CMP) on the effective soil fertility, mineral nutri...
THE FEATURES OF INTERACTION RHIZOBIUM GALEGAE WITH GALEGA ORIENTALIS
The strain of Rhizobium galegae К- 3 - active symbiote of Galega orientalis has been received by means of selection. Positive influence of this strain on formation and functioning of symbiosis during the first and second...