Expression and Purification of Nisin in Escherichia coli
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 4
Abstract
Fusion expression is a promising strategy for the production of bioactive peptides in Escherichia coli to enhance either soluble protein level or purification potential. Nisin is the bacteriocin that had been extensively studied and had been widely applied in many areas such as food and pharmaceutical. However, scientific reports on recombinant nisin production in E. coli are still insufficient. In this study, we constructed a new expression plasmid containing the coding sequence of NusA, hexahistidine and Lactobacillus lactic nisin coding sequence. Next, we introduced the expression plasmid into BL21 E. coli and produced the recombinant fusion nisin in E. coli. Recombinant E. coli extract was purified by nickel affinity chromatography and resulted in 77% yield with 55% purity. The bioactive nisin was successfully released from NusA 6xHis Nisin fusion protein by the endonuclease. The nisin showed its antibacterial activity on Listeria monocytogenes with an activity unit of 18.9 AU/mg. The nisin bioactivity has stable at the temperature range of 30–90oC and in pH range of 1–12. The results showed that the new construction was appropriate for production of nisin bioactive peptides.
Authors and Affiliations
Huynh Thi Xuan Mai, Nguyen Van Hau, Nguyen Hieu Nghia, Dang Thi Phuong Thao
Keywords
Related Articles
- EP ID EP671026
- DOI 10.21276/ijlssr.2018.4.4.9
- Views 102
- Downloads 0
Microbial, Hydrobiological Indicators and Physicochemical Characteristics of a Remote Aviation Fuel-Contaminated Lentic System in Ibeno, Nigeria
This research paper presented the microbial and hydrobiological indicators and the physicochemical quality of water samples from a lentic ecosystem in Ibeno LGA, Nigeria, after sixteen (16) years of an aviation fuel spil...
Yield of Potato (Solanum tuberosum L.) as Influenced by Variety and Planting Date in the Sudan Savanna Ecological Zone of Nigeria
Potato is a temperate crop requiring low temperatures for growth and yield. Production of potato in the Sudan savanna agro-ecological zone of Nigeria is restricted to periods of low temperatures that prevailed from earl...
Mutilated Occlusion Fixed-Removable Approach- A Case Report
Partial or complete edentulism has multiple implications in relation to function, esthetics and future rehabilitative treatment. This case report illustrates the management of a patient with extreme consequences of parti...
Role of Oxidant Alteration of Biomolecules in Diabetes and Other Associated Diseases
Reactive oxygen species (ROS) are products of normal cellular metabolism and are known to act as second messengers. Physiological conditions, ROS participate in maintenance of cellular ‘redox homeostasis’ in order to pro...
Psychiatric Co-morbidities and Management Outcomes in Mentally Ill Prisoners
Background: The occurrence of psychiatric disorders is more in the prisoners than in general population. Co-morbidity is seen to be an important and complex entity in clinical assessment of mental state competence (dimin...