Extraction and purification of ßeta-galactosidase from a Local Almond seeds(Prunusamygdalus)
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 1
Abstract
ßeta-galactosidaseenzyme is greatly dispersed in plant tissues and it’s known to be implicated in hydrolyzing terminal non reducingß.D.Galactosyl residues from polysaccharides . This study involved the selection of the best extraction of the enzyme among seven methods.The phosphate buffer (pH7) had given a highest activity of crude enzyme was 8.85 U. The aqueous crude of almond seeds was used to detect of some compounds by phytochemical screening tests and using Thin layer chromatography(TLC). The protein content was concentrated and precipitated by ammonium sulphate (0-40)% as apartial purification. The purification stages of crude enzyme were achieved by using gel filtrationchromatography (sephadex-75) at a yield of (136.4)% with activity was 4.4 u/ml . followed by ion-exchange column chromatography (DEAE-cellulose A52) and five iso-enzymes were obtained at a yield of 93% with 4.6 times of purification fold for iso-enzyme (I) while 49.6% and 4.2 times of iso- enzyme (V).
Authors and Affiliations
SanadBaqerMohhamedJinan Hussein Murtadha, ImanHashimAbdul Razzaq
Keywords
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