Extraction, Isolation and Antimicrobial Activity of Crude and Purified Ferritin extract from seeds of Soyabean (Glycine max (L.) Merr. )
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 2
Abstract
The present study aimed to isolate, purify and evaluate the antimicrobial activity of ferritin extracted from Soyabean (Glycine max (L.) Merr. ) against Gram-negative microorganisms (Escherichia coli, Pseudomonas aeruginosa, Kliebsiella Pneumonia, Proteus vulgaris), as well as Gram-positive microorganism (Staphylococcus aureus, Staphylococcus epidermis) and fungus Candida albicans. Agar well diffusion method was adapted to determine antibacterial activity against all the test microorganisms. Zone of inhibition of the crude and pure extracts were tested. Among all the test pathogens E. coli was found susceptible with zone of inhibition 9mm. to the crude and purified ferritin extract. The present study successfully isolated and purified the single unit of Glycine max (L.) Merr. ferritin with 28- kDa units . It also reveals the least susceptibility of the microorganisms towards the ferritin isolated from seeds of Glycine max (L.) Merr. Keywords: Ferritin, Glycine max (L.) Merr. , Antimicrobial activity
Authors and Affiliations
Supriya Kapil Shetty Thanekar, Ramachandra Y L, Udgire M
Keywords
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