Extraction of lipids and purification of linoleic acid from Clarias macrocephalus oi
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 3
Abstract
Fatty acid composition of “keli” (Clarias macrocephalus) oil has been shown to contain 18.4% of linoleic acid (LA). Oil was obtained from three types of extraction methods namely Blight & Dryer method, Soxhlet method, and pressing method. Blight & Dryer method produced an acceptable total lipid content with good quality of oil. After saponification and acidification, purification of released LA by urea complex with urea to fatty acid ratio 5:1 and crystallization temperature of 20°C, had enhanced purity of LA to 47% with total fatty acid recovery of 25.2%. The LA obtained was converted to LA methyl ester prior to further purification by argentated silica gel chromatography. LA with a purity of 92% with total fatty acid recovery of 12.9% was obtained.
Authors and Affiliations
Noorul Zainuddin, Abdul Babji, Mamot Said
Keywords
Related Articles
- EP ID EP113867
- DOI -
- Views 97
- Downloads 0
Distribution and community structure of fish in Obitsu-gawa River Estuary of inner Tokyo Bay, central Japan
The distribution and community structure of fish in Obitsu-gawa River Estuary of inner Tokyo Bay, central Japan was studied from May to December 2005 and March to April 2006. A total of 19,006 individuals, represented by...
Rapid population collapse of<br /> the critically endangered Valencia letourneuxi in Kalamas basin of Northwest Greece.
In order to assess the current status of the critically endangered Valencia letourneuxi in Kalamas River basin, an extensive field survey including twelve sampling stations was undertaken using back-pack electrofishing d...
Mixed stock analysis (MSA) - a tool for proper management in fisheries
For natural environment, it is crucial to develop a fishery management plan, outlining conservation and restoration measures. From this point of view, if the fishery is managed as a single unit (stock), there is a huge p...
Evaluation of seasonal variations in physicochemical parameters of Taleghan River, northern Iran
In this study an experiment on water quality of Taleghan River between August 2008 and July 2010 was conducted. Twenty four samples from 6 stations along the river were taken monthly. Parameters like temperature, dissolv...
Zeroes do matter: the tale of the missing fishes in Cabilao Island Bohol, Philippine
Data from plankton surveys typically contain many zeroes. Pomacentrid larvae collected from the reef of Cabilao Island, Bohol, Philippines was typical of such data wherein 294 out of 354 tows had 0 larval counts. This...