Fibrin Clot Adhesion To Instrumented Conditioned Root Surfaces By MTAD And EDTA- A SEM Study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 10
Abstract
Background: Smear layer removal and formation of stable bond between blood clot and root surface is desirable for periodontal healing, which can be achieved by using root biomodifiers. The objective of this invitro study was to evaluate the root surface changes in terms of fibrin clot adhesion subsequent to the application of MTAD and EDTA. Material and methods: Thirty human single rooted teeth were collected and grouped in to Saline (control Group-I), 15% EDTA gel (Group-II), MTAD Biopure (Group- III) treatment groups containing 10 teeth in each group. After root conditioning, fresh human blood was applied to each root specimen and was allowed to clot, subsequently samples were dehydrated in graded series of ethanol and visualized under Scanning electron microscope. Results: The highest blood clot attachment score was observed with roots treated with MTAD followed by EDTA and least results was observed with control group saline. Conclusion: Specimens treated with MTAD best supported the fibrin clot attachment. EDTA appeared to be less effective in supporting the clot. Saline was least effective of all the samples, showing presence of smear layer.
Authors and Affiliations
Dr Gaurav Didhra, Dr Jagjit Singh, Dr Parveen Dahiya, Dr Sachin Upadhyay, Dr Deepak Phor, Dr Ankur Sharma
Keywords
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