Field response of Dioscorea alata L. clone ‘Caraqueño’ in vitro plants at different times of plantation
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 3
Abstract
The use of biotechnological methods of in vitro plant tissue culture to produce categorized seed yams (Dioscorea spp.) has allowed increased agricultural yields of this crop. This study aimed to determine the response of in vitro plants of Dioscorea alata L. clone ‘Caraqueño’ at different times of planting in the field. The treatments consisted in field planting of previously acclimatized in vitro plants, from June to November, once a month. In June were planted 125 g fresh weight fragments tubers as a control. After 30 days of culture in all the treatments the survival of plants was evaluated and at the time of harvest (January) 20 plants per treatment were taken and determined agronomic variables such us number of tubers per plant, fresh mass of tubers per plant (kg), tuber fresh weight (kg) and tuber dried mass (%). The results showed that the in vitro plants reached high survival rates (96.5 to 99.5%), with higher values for the number and fresh weight of tubers per plant in the months from June to August (3.3, 3.1 and 2.9), and June and July (3.5 and 3.1 kg), respectively in relation to the conventional plant material propagation. It was demonstrated that in vitro plants can be planted in field from June to November, with production of tubers that can reach the category of commercial (June to August) and the category of basic seed (September to November).
Authors and Affiliations
Misterbino Borges García , Rafael Gómez-Kosky , Edil Estrada Abeal , Diana Reyes Avalos , Bernard Malaurie , Reisel Destrada Batista
Keywords
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