First molecular identification of Cryptosporidium by 18S rRNA in goats and association with farm management in Terengganu
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 5
Abstract
Objective: To identify the prevalence of Cryptosporidium from goats in three types of farm management systems in Terengganu, Malaysia and to determine the Cryptosporidium species infecting goats by using 18S rRNA. Methods: A total of 478 fecal samples were randomly collected from goats in three farms; 199 samples were collected from intensive farm, 179 samples from semi-intensive farm and 100 samples from extensive farm. The samples were processed by using formolether concentration technique and stained by using modified Ziehl–Neelsen. Positive samples were performed by using nested PCR analysis by using 18S rRNA. Results: Out of 478 goats, 207 (43.3%) were found to be infected with Cryptosporidium. Goats reared under the intensive farm management system reported the highest prevalence of infection (49.7%), followed by intensive farm management system (41%) and the lowest prevalence was reported in the goats reared under semi-intensive management system (37.4%). Conclusions: The identified species found in goat was Cryptosporidium parvum. Future study on the zoonotic transmission of Cryptosporidium parvum in goats needs to be done in order to find the source of transmission of this parasite.
Authors and Affiliations
Afzan Mat Yusof, Najat Hashim, Muhammad Lokman Md Isa
Keywords
Related Articles
- EP ID EP253657
- DOI 10.1016/j.apjtb.2017.01.008
- Views 75
- Downloads 0
Effective Aeromonas specific monoclonal antibody for immunodiagnosis
Objective: To identify the monoclonal antibody specific to Aeromonas spp., a Gram negative bacteria causing gastroenteritis and wound infection. Methods: The monoclone, namely 88F2- 3F4, was produced from hybridoma techn...
In vivo study of pomegranate (Punica granatum) peel extract efficacy against Giardia lamblia in infected experimental mice
Objective: To investigate the efficacy of pomegranate (Punica granatum) peel extract as an alternative treatment on the white laboratory mice against giardiasis. Methods: Experimental animals were divided into five group...
In vitro antimicrobial activity of mangrove plant Sonneratia alba
Objective To investigate the antimicrobial property of mangrove plant Sonneratia alba (S. alba). Methods The antimicrobial activity was evaluated using disc diffusion and microdilution methods against six microorganis...
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene
Objective: To investigate the presence, prevalence and phylogenetic classification of bovine torovirus (BToV). Methods: Stool samples from 72 calves, which were negative for primary gastroenteritis agents, were examined...
Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E
Objective: To prove the molecular mechanisms of Mahkota Dewa (Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression. Methods: In th...