Flow cytometry: an overview in optical system and application in biological studies
Journal Title: European Journal of Biological Research - Year 2016, Vol 6, Issue 3
Abstract
Flow cytometry is a technology that measures simultaneously both optical and fluorescence properties of single cells or any other particle including cell organelles (i.e. nuclei, chromosome etc.), microorganisms, and latex beads. This technology also exploits bright beams of light (usually from laser) to directly disclose various aspects i.e. size, granularity (internal complexity of cell) including relative fluorescence intensity of cells by the way of light is scattered or indirectly by coupling wth fluorescent probes to cell components or suspended particle. Some components like dichroic mirror, optical filter, photomultiplier tube, detectors etc. that played a crucial role in flow cytometry. Therefore, light scattering properties and measurement of fluorescence through flow cytometry is widely used in life science laboratories including preclinical and clinical research for many applications such as immunological, entomological, pharmaceutical etc. This review provides information about the optical system that are used in flow cytometry and showed various biological applications.
Authors and Affiliations
Amit Gupta, Deepak Vakhare, Sushama R. Chaphalkar
Keywords
Related Articles
- EP ID EP230362
- DOI 10.5281/zenodo.59277
- Views 128
- Downloads 0
The decline of the white-tailed jackrabbit (Lepus townsendii): carbohydrate and soil texture analysis
A decline of the white-tailed jackrabbit, (Lepus townsendii), has been occurring throughout the species natural range. This has provoked the need for research and a greater understanding of the reasons behind the decline...
Effect of mareb crude oil, dispersed oil and dispersant (OSD) on filtration rates of the clams Tivela ponderosa under laboratory conditions
This study includes laboratory experiments of filter-feeding activity of bivalves Tivela ponderosa. The filtration rate of bivalves Tivela ponderosa was controlled hourly at 5th and 10th day (every two and six hours). Th...
Immunosuppressive and cytotoxic potential of aqueous leaves extract of Thlaspi arvense
Medicinal plants are traditionally used in Asian countries for treatment of various intracellular as well as extracellular infections. The present study was undertaken to investigate Thlaspi arvense for determining its i...
Enzyme producing capabilities of some extremophilic fungal strains isolated from different habitats of Wadi El-Natrun, Egypt. Part 1: Protease, lipase and phosphatase
Thirty-nine isolates of the most commonly encoun-tered fungal species from different extreme habitats of Wadi El-Natrun region were tested for their capabilities of producing protease, lipase and phosphatase enzymes. Mos...
Winter wheat yield and soil physical properties responses to different tillage and irrigation
Adopting a better tillage system not only improves the soil properties and crop productivity but also improves water use efficiency. A field experiment was conducted over 2 years in Norman E Borlaug Crop Research Centre...