FREE RADICAL SCAVENGING INDEX OF CUCURBITA MAXIMA SEEDS AND THEIR LIBS BASED ANTIOXIDANT ELEMENTAL PROFILE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 4
Abstract
Objective: To assess the free radical scavenging index and antioxidant capacity of C. maxima (Cucurbita maxima) seed extract in vitro and its correlation with the phytoelemental profile, quantitatively assessed by LIBS (Laser-Induced Breakdown Spectroscopy).Methods: A number of in vitro antioxidant and free radical scavenging assays were performed along with standard as a reference. Reduction capacity and scavenging potential of C. maxima seeds were analyzed in addition to their IC50 values and were compared with respective standards. Quantitative measurements of phytoelements present which is responsible for antioxidant activity is carried out by LIBS.Results: Significant Antioxidant power of the extract was validated by high values of Total Phenolics (18.42 mg/g of gallic acid equivalent) and Total Flavonoids (5.53 mg/g of quercetin equivalent). A result of Ferric Reducing Antioxidant Power (163.4 μM Fe+2/g) clearly reflects its reduction capacity. Percentage inhibition of 2,2-diphenyl-1-picrylhydrazyl (69.25), Nitric Oxide (63.63) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (62.09) radicals proves its antioxidant potential undoubtedly, which was further confirmed by their IC50 values. Reducing power was found to increase significantly in a dose-dependent manner. Extent lipid peroxidation was also measured by comparing the results of Ferric thiocyanate assay with the Thiobarbituric acid method. LIBS analysis showed the presence of certain phytoelements viz. Mg, Ca and Na in the extract, which could be responsible for its antioxidant capacity.Conclusion: Thus, the seeds of C. maxima having a combination of antioxidant phytochemicals and phytoelements could be explored for developing as a complementary and alternative medicine for managing oxidative stress including diabetes as well as aging.Keywords: Reactive oxygen species, Cucurbita maxima, Elemental analysis, Laser spectroscopy
Authors and Affiliations
Devesh Kumar Kushawaha, Manjulika Yadav, Sanjukta Chatterji, Gulab Singh Maurya, Awadhesh Kumar Rai, Geeta Watal
Keywords
Related Articles
- EP ID EP577744
- DOI -
- Views 60
- Downloads 0
THERAPEUTIC POTENTIAL OF LICHEN PARMELIA PERLATA AGAINST DUAL DRUG-RESISTANT HELICOBACTER PYLORI ISOLATES
Objective: H. pylori have acquired resistance to the commonly used antibiotics due to their use in many anaerobic and parasitic infections leading to treatment failure of various gastroduodenal diseases associated with H...
COMPARATIVE PHARMACOGNOSTICAL AND PHYTOCHEMICAL STUDY OF VARIOUS SPECIES OF THE CONTROVERSIAL DRUG ‘PUNARNAVA'
Objective: Punarnava is a controversial drug i.e. its true botanical identity has not been ascertained, and several different species are used as Punarnava. It is a part of many medicinally important traditional formulat...
IN VITRO EVALUATION OF GLYCOLIPOPROTEIN POWDER FROM EARTHWORM EUDRILUS EUGENIAE
Objective: The researchers are searching for innate bioactive compounds, competent of curing various diseases. In the present study, the cytotoxicity and the angiogenic nature of the glycolipoprotein powder from the eart...
EFFECTS OF OLIVE DRYING AND STORAGE ON THE OXIDATIVE STATUS, AROMA, CHLOROPHYLL AND FATTY ACIDS COMPOSITION OF OLIVE OIL
Objective: In this study, we thoroughly investigate the effect of drying and storage of olives from four Tunisian cultivars (Chetoui, Chemlali, Oueslati and Picholine) on the final composition of olive oil.Methods: Olive...
APPROACHES TO ACHIEVE AN ORAL CONTROLLED RELEASE DRUG DELIVERY SYSTEM USING POLYMERS: A RECENT REVIEW
The present article contains a brief review of various formulation approaches used in controlled release drug delivery systems, the role of polymers in the controlled delivery of many fast release drugs and the mechanism...