Frequency of chromosomal aneuploidy in high quality embryos from young couples using preimplantation genetic screening
Journal Title: International Journal of Reproductive BioMedicine - Year 2017, Vol 15, Issue 5
Abstract
Background: Selection of the best embryo for transfer is very important in assisted reproductive technology (ART). Using morphological assessment for this selection demonstrated that the correlation between embryo morphology and implantation potential is relatively weak. On the other hand, aneuploidy is a key genetic factor that can influence human reproductive success in ART. Objective: The aim of this lab trial study was to evaluate the incidence of aneuploidies in five chromosomes in the morphologically high-quality embryos from young patients undergoing ART for sex selection. Materials and Methods: A total of 97 high quality embryos from 23 women at the age of 37or younger years that had previously undergone preimplantation genetic screening for sex selection were included in this study. After washing, the slides of blastomeres from embryos of patients were reanalyzed by fluorescence in-situ hybridization for chromosomes 13, 18 and 21. Results: There was a significant rate of aneuploidy determination in the embryos using preimplantation genetic screening for both sex and three evaluated autosomal chromosomes compared to preimplantation genetic screening for only sex chromosomes (62.9% vs. 24.7%, p=0.000). The most frequent detected chromosomal aneuploidy was trisomy or monosomy of chromosome 13. Conclusion: There is considerable numbers of chromosomal abnormalities in embryos generated in vitro which cause in vitro fertilization failure and it seems that morphological characterization of embryos is not a suitable method for choosing the embryos without these abnormalities.
Authors and Affiliations
Farzaneh Fesahat, Fatemeh Montazeri, Mohammad Hasan Sheikhha, Hojjatollah Saeedi, Razieh Dehghani Firouzabadi, Seyed Mehdi Kalantar
Keywords
Related Articles
- EP ID EP183854
- DOI -
- Views 132
- Downloads 0
Macroscopic features of the kidneys of fetuses and newborns in preeclampsia: postmortem observational study
Background: The state of the mother health is of great importance in the development of children renal pathology. Objective: To reveal macroscopic features of the fetuses and newborns kidneys of the mothers whose pregnan...
New single nucleotide polymorphism G5508A in the SEPT12 gene may be associated with idiopathic male infertility in Iranian men
Background: Male infertility is a multifactorial disorder, which affects approximately 10% of couples at childbearing age with substantial clinical and social impact. Genetic factors are associated with the susceptibilit...
Comparison of lifestyle in fertile and infertile couples in Kermanshah during 2013
Background: Infertility is a major reproductive health in gynecology. According to the world health organization, there are currently 50-80 million infertile couples in the world. Objective: Considering the critical effe...
Effect of metformin on maternal and neonatal outcomes in pregnant obese non-diabetic women: A meta-analysis
Background: Metformin reduces maternal and neonatal weight gain in gestational diabetes mellitus; however, this effect is poorly investigated in non-diabetic women. Objective: We performed this meta-analysis to investiga...
Evaluation of the metformin effects on Anti-Mùˆllerian Hormone in women with polycystic ovarian syndrome: A double-blind randomized clinical trial
NA