Functional and structural state of rats’ kidneys and liver under the influence of nano-polymer based on pseudopolyamino acids
Journal Title: Біологія тварин - Year 2016, Vol 18, Issue 3
Abstract
The article presents results of research of the influence of nano-polymer based on pseudopolyamino acids named GluLa-DPG-PEG600 on structural and functional state of kidneys and liver of Rattus norvegicus var. alba. This article precisely describes the influence of GluLa-DPG-PEG600 on activity of alanine transaminase (ALAT), aspartate transaminase (ASAT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGTP) and content of cholesterol and creatinine in blood, — moreover histological analysis of rats liver and kidneys was prepared. We found that single injection of GluLa-DPG-PEG600 caused increasing activity of ALAT, ASAT, ALP, GGTP and amount of creatinine in blood of rats on the 7th day of experiment. We observed decreased level of enzymes and content of cholesterol and creatinine on the 14th day of experiment comparing with results obtained on the 7th day of experiment. On the 21st day of experiment activity of all enzymes and content of creatinine and cholesterol returned to the same level of activity as in the control group animals.Histological analysis revealed few inflammation processes in ascending convoluted tubules of nephrons on the 7th and 14th days of experiment that confirms increased enzyme activity and content of creatinine and cholesterol. In deed on the 21st day of experiment histological structure of rats and liver were unchanged. Our results show temporary little toxic effect of GluLa-DPG-PEG600 on rats on the 7th and 14th days of experiment. Decreased toxic effect on the 14th day and its absence on 21st day of experiment can be explained by adaptation process to repeated injections of GluLa-DPG-PEG600 in rats. In conclusion GluLa-DPG-PEG600 could be used as carrier of active ingredients of drugs for future research.
Authors and Affiliations
B. O. Chekh, M. V. Ferens, Yu. V. Martyn, D. D. Ostapiv, V. V. Vlizlo
Keywords
Related Articles
- EP ID EP261265
- DOI 10.15407/animbiol18.03.107
- Views 109
- Downloads 0
Influence of feed additive “Humilid” on protein and amino acid metabolism indicators in broiler chickens of Cobb 500 cross
The article presents data on the effect of the feed additive “Humilid” the general biochemical parameters of blood and liver in broiler chickens of the Cobb 500 cross. It has been shown that chickens which received “Humi...
Effect of glucose and partial dehydration on resistance of mammalian erythrocytes to hypertonic shock
The paper is devoted to the studying the resistance of human, rabbit and rat erythrocytes to the effect of hypertonic shock when changing an initial status of cells modified with glucose and 0.4 mol/l NaCl. The hemolysis...
Histological structure of separate internal organs of piglets at application of probiotic feed additive
The aim of the test was to study the influence of probiotic feed additive in doses recommended by the producer and duration of application on the histological structure of separate internal organs of piglets. The product...
Development of technology of blood coagulation factor VIII purification by the method of affinity chromatography on silica sorbents
The manufacturing processes of chromatographic purification are used increasingly for plasma fractionation in the last few years. Implementation and combination of ion-exchange, affinity and size-exclusion chromatography...
The PTTG1 is a novel inhibitor of osteogenic differentiation of mouse mesenchymal stem cells
Rheumatoid arthritis (RA) is a severe autoimmune inflammatory disease leading to chronic pain in the joints frequently ending in patient disability and even death. Molecular mechanisms that trigger a disease and exacerba...