Gamma and UV radiation induced mutagenesis in Trichoderma reesei to enhance cellulases enzyme activity
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 5
Abstract
The need for renewable energy resources has focused attention on the renewable polysaccharide cellulose, which can be enzymatically hydrolyzed to yield sugars. The filamentous fungus Trichoderma reesei is an important host organism for industrial enzyme production producing large amounts of hydrolytic enzymes. The aim of the present study was to improve the cellulase production of T. reesei through a successive mutagenesis step. Trichoderma reesei (PTCC 5142) a cellulolytic microorganism, was subjected to mutagenesis using gama and UV radiation individually as mutagens. Successive mutants showed rapid growth on plates containing MYG medium, clear hydrolysis zone on mandels medium and enhanced cellulase production. The mutant strains of T. reesei were isolated and selected by mandel screening method. Results showed some mutant strains has had higher cellulases activities in comper with parent strain. A mutant strain of Trichoderma reesei (PTCC 5142) with maximum production potential of cellulase was finally selected. The developed mutant from gama mutagenesis way is the best mutant for Ccase, CMCase, Avicellase and Fpase activities. This new method could be applied to obtain potent fungal mutants for more enzymes production.
Authors and Affiliations
Samira Shahbazi, Khodayar Ispareh, Mahsa Karimi, Hamed Askari and Mohammad ali Ebrahimi
Keywords
Related Articles
- EP ID EP32598
- DOI -
- Views 304
- Downloads 0
Effect of fertilizer and cover crop on yield and yield components of soybean
In order to study the effect of fertilizers and cover crop on yield and yield components of soybean, an experiment was conducted in Research field of Islamic Azad University of Shirvan in 2014 growing season. Treatment...
Drift in sprayers and effects of weather conditions on drif
Attention to the safety and environmental issues in all sectors of agriculture, industry and services in different countries is important. In the agriculture, each year many efforts has been done to find alternatives m...
High-performance liquid chromatography (HPLC) liquid chromatography-mass spectrometry (GC/ MS) analysis of some Sudanese propolis
Propolis is a natural resin of a variety of plant origins collected by honeybees to seal gaps and cracks in their hives. Propolis was used as a remedy of various diseases due to its versatile biological activities. At...
Effect of salicylhydroxamic acid (SHAM) and pyridoxine on bioactive compounds and enzyme activity of germinated wheat
This research was conducted in order to study the effect of priming on wheat seed (Triticum aestivum L.) by pyridoxine and salicylhydroxamic acid (SHAM) on germination and development characteristics as factorial desig...
Evaluation of pre-seeding herbicides in wheat crop
Effective wild oat control with pre-emergent herbicides is necessary in minimum tillage systems. Pre-seeding herbicides do not work as well in minimum tillage systems compared to systems using full cut cultivation wher...