GC–MS analysis and anti–mosquito activities of Juniperus virginiana essential oil against Anopheles stephensi (Diptera: Culicidae)
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2019, Vol 9, Issue 4
Abstract
Objective: To investigate phytochemicals present in the essential oil from aerial parts of eastern red cedar, Juniperus virginiana (J. virginiana) L. (Cupressaceae) and to determine its killing and repellent activities against larvae, pupae, and adults of the Asian malaria mosquito, Anopheles stephensi (Diptera: Culicidae). Methods: J. virginiana essential oil was extracted by hydrodistillation, and its chemical composition was determined by gas chromatography-mass spectrometry. Seven different logarithmic concentrations of J. virginiana essential oils were used in larvicidal and pupicidal assays. J. virginiana essential oils-impregnated bed nets were applied in a designed animal module to test excito-repellent activity against adult mosquitoes. Results: Fourteen constituents corresponding to 99.98% of J. virginiana essential oils were identified. Five main components were terpinen-4-ol (25.21%), camphor (19.89%), E-3-hexen- 1-ol (13.30%), γ-terpinene (7.86%), and l-menthone (2.27%). The LC50 and LC90 values against larvae of the Anopheles stephensi were 11.693 and 66.140 ppm and for pupae were 9.640 and 40.976 ppm, respectively. In excito-repellency assay, J. virginiana essential oils-impregnated bed nets provided an average of 54.63% protection for guinea pig and 45.37% mortality for the mosquitoes. Conclusions: Four monoterpenes and one leaf alcohol were identified by gas chromatography-mass spectrometry. J. virginiana essential oils showed potent larvicidal, pupicidal, adulticidal, and repellent activities against Anopheles stephensi at acceptable concentrations. Evaluation of bioactivity of identified chemicals (alone or in combination) will provide new eco-friendly substances for mosquito-management programs.
Authors and Affiliations
Azar Tahghighi, Naseh Maleki-Ravasan, Navid Dinparast Djadid, Hamzeh Alipour, Raziyeh Ahmadvand, Fateh Karimian, Saeed Yousefinejad
Keywords
Related Articles
- EP ID EP540960
- DOI 10.4103/2221-1691.256730
- Views 82
- Downloads 0
Nigella sativa protects against isoproterenol-induced myocardial infarction by alleviating oxidative stress, biochemical alterations and histological damage
Objective: To evaluate the cardioprotective effect of Nigella sativa L. (N. sativa) in isoproterenol-induced myocardial infarction (MI). Methods: Groups were treated with different doses of ethanol extract of N. sativa (...
Phytochemical constituents, antioxidant and antibacterial activities of methanolic extract of Ardisia elliptica
Objective: To investigate the antioxidant, antibacterial, and chemical ingredients of Ardisia elliptica (A. elliptica) methanolic extracts. Methods: The plant was extracted using methanol. Antibacterial and antioxidant a...
Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack
Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated...
Chemical composition of essential oil of Thymus vulgaris collected from Saudi Arabian market
Objective: To focus on the analysis of chemical constituents of the Thymus vulgaris L. (locally known as “Zaitra” or “Za'atar”; Family: Lamiaceae) which is available in the market of Saudi Arabia. Methods: The Zaitra oil...
Immunomodulatory activity of butanol fraction of Gentiana olivieri Griseb. on Balb/C mice
Objective To explore the immunomodulatory properties of 80% ethanol extract and butanol fraction of Gentiana olivieri (G. olivieri) Griseb on Balb/C mice. Methods The study was performed with basic models of immunomod...