GC-MS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF SUDANESE ALLIUM CEPA L. FIXED OIL
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 6
Abstract
The present study was undertaken to identify and quantify the chemical constituents of seed oils from two cultivated varieties of Allium cepa – red onion and white onion and to evaluate their potential antimicrobial activity. GC-MS analysis showed 27 components for red onion. Major constituents are: 9,12-octadecadienoic acid methyl ester(42.45%), 9-octadecenoic acid methyl ester(19.44%), hexadecanoic acid(11.33%), cyclpentaneoctanoic acid methyl ester( 6.82%) and methyl stearate(5.06%). The white onion gave 24 constituents being dominated by: 9,12-octadecadienoic acid methyl ester(55.29%), 9-octadecenoic acid methyl ester(16.07%), hexadecanoic acid(12.70%) and methyl stearate(4.91%). The antibacterial activity of the oils was assessed via cup plate agar diffusion assay against six standard human pathogens. The oil from red onion showed different antimicrobial responses against test organisms. It showed excellent activity against the bacterial strains: Staphylococcus aureus and Bacillus subtilis. It also exhibited significant activity against the yeast Candida albicans. However, it was inactive against Pseudomonas aeruginosa, Escherichia coli and the fungal species Aspergillus niger. White onion oil showed excellent activity against Escherichia coli. It also showed significant activity against Staphylococcus aureus and Bacillus subtilis.
Authors and Affiliations
Dr. Abdel Karim M.
Keywords
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