GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS AND ANTIMICROBIAL ACTIVITY OF CRYPTOCOCCUS RAJASTHANENSIS KY627764 ISOLATED FROM BOMBYX MORI GUT MICROFLORA.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 3
Abstract
The insect gut microflora acts as a large reservoir of bioactive natural products as a diversity of resident microflora is found to be symbiotically associated with the insect. The present study reports the bioactive chemical compounds and the antimicrobial nature of the yeast isolated from the insect Bombyx mori gut. The yeast is identified as Cryptococcus rajasthanensis by Molecular characterization. The Fourier transform infrared spectroscopy (FT-IR) studies of the chloroform and ethyl acetate crude yeast extracts indicated a number of functional groups like alcoholic, phenolic, ester, aldehydic, etc. that accounts for the bioactive nature of the extracts. The Gas chromatography-Mass spectroscopy (GC-MS) analysis of the crude extracts revealed a large number of bioactive compounds of high and low molecular weight that are considered biologically active. Some of the biologically active molecules like phenol 2,4-bis(1,1-dimethylethyl), 1,2-benzenedicarboxylic acid dibutyl ester, celidoniol deoxy, nonadecane, tetratetracontane, 2-methyloctacosane and pentadecane bearing antimicrobial property were detected in both chloroform and ethyl acetate extracts. Furthermore, the crude extracts were subjected to antimicrobial assay by agar well diffusion method and are found to be antimicrobial against four pathogenic bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa and two fungi Candida albicans and Aspergillus flavus. The chloroform extract showed maximum inhibition for E. coli and minimum for A. flavus.
Authors and Affiliations
Delicia Avilla Barretto, Shyam Kumar Vootla.
Keywords
Related Articles
- EP ID EP281039
- DOI 10.21474/IJAR01/6700
- Views 75
- Downloads 0
ROLE OF MALONDIALDEHYDE(MDA) AND NITRIC OXIDE (NO) IN PATIENTS WITH BREAST CANCER DISEASES.
Background: Breast cancer is a malignant tumor that begins in the breast cells . Malignant tumor is a group of cancer cells that able to grow into surrounding tissues or spread (metastasize) to faraway regions of the bod...
A NOVEL SCHEME FOR CONTINUOUS MONITORING OF KIDNEY PATIENTS.
Internet of things is been a major field of research in smart health care system due to its vast advantages like keeping a detailed record of patient health , remote sharing of data and its ability to connect with cloud...
IN VITRO EVALUATION OF ESSENTIAL OILS AGAINST RHIZOCTONIA SOLANI (KUHN) THE CAUSAL OF SEEDLING DAMPING OFF DISEASE.
Fourteen fungal isolates were isolated from infected tomato roots from Jazanrejion, Southern of Saudi Arabia (KSA) on potato dextrose agar (PDA) medium and corn meal agar (CMA). Among the isolates the most serious soil a...
PREVELANCE OF DOMESTIC INJURIES AND FIRST AID AWARENESS AMONG KUWAITS 18 OLD AND ABOVE.
Injury is defined medically as being harm as a result of trauma; it is one of the most common problems that may face people in their daily life especially at home. Domestic injuries may sometimes be life-threating issue...
LES DESORDRES DE DEVELLOPEMENT SEXUELS (DSD) : UNE PROBLEMATIQUE DE PRISE EN CHARGE.
Les diff?rentes anomalies du d?veloppement sexuel (DSD) regroupent un ensemble d?atteintes dont le degr? de gravit? et les cons?quences sont variables. La d?couverte d?une anomalie des organes g?nitaux sur les ?chographi...