Genetic analysis of [i]Apis mellifera[/i] macedonica (type [i]rodopica[/i]) populations selectively reared for purposive production of honey bee queens in Bulgaria
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 1
Abstract
The genetic polymorphism in selectively reared in Bulgaria, local honey bee populations of [i]Apis mellifera macedonica[/i] subspecies (type [i]rodopica[/i]), has been studied, using analysis of six enzyme systems (MDH-1, ME, EST-3, ALP, PGM and HK) corresponding to 6 loci. Totally 458 worker bees from 12 bee breeding bases for artificially inseminated queens were used for this study. All these stations are part of the National Bee Breeding Association which officially implements a National Program for sustainable beekeeping in Bulgaria. All of the six loci were found to be polymorphic. Only EST-3 locus was established as fixed in one of the investigated populations. Polymorphism with three alleles was ascertained for MDH, ME, ALP, PGM and HK loci and with five alleles for EST-3 locus. The most common alleles in almost all of the populations were MDH-1 100, ME 100, EST-3 100, PGM 100 and HK 100. Two private alleles (frequency < 0.05) were found for two of the populations. The calculated level of polymorphism was 88.33% in only one of the populations and 100% - in all others. The observed and expected heterozygosities (Ho and He) ranged from 0.157 to 0.250 and from 0.206 to 0.272, respectively. The estimated mean FST value from allozyme data was 0.035. On the bases of the allele frequencies of the studied allozyme loci the Nei's (1972) genetic distance was estimated. It ranged between 0.002 and 0.060 among the populations studied.
Authors and Affiliations
Vida Georgieva, Plamen Petkov, Nikolay Petkov, Evgeniya Ivanova
Keywords
Related Articles
- EP ID EP112315
- DOI -
- Views 93
- Downloads 0
Evaluation of salinity tolerance in maize (Zea mays L.) genotypes at seedling stage
Reproducible and reliable evaluation methods are the basis of any successful breeding programme especially for abiotic breeding. In this study, solutionculture and vermiculite-culture methods were employed to evalu...
Aberrant signature patterns of ATM, γ-H2AX and p53 proteins in the patients exposed to methyl isocyanate diagnosed with gallbladder cancer
Cancer of gallbladder is a hidden phenomenon and highly malignant with underprivileged diagnosis and poor survival. Study of cancer patterns amongst victims of Bhopal gas tragedy exposed to methyl isocyanate reveal...
Influence of composition on the biological activity of pectic polysaccharides from leek
Water-soluble and acid-soluble pectic polysaccharides with significant immunostimulating activity were isolated from alcohol-insoluble solids (AIS) of leek. Pectic polysaccharides characterized with high anhydrouronic ac...
Purification and characterization of hyaluronic acid produced by Streptococcus zooepidemicus strain 3523-7
Hyaluronic acid (HA) is a hydrated gel and comprises repeating units of glucuronic acid and N-acetylglucosamine. Production and recovery of HA has gained great importance due to its vast clinical applications. In pursuit...
Phytochemical analysis and antimicrobial activity of baobab (Adansonia digitata) leaves and steam bark extracts on Staphylococcus aureus and Escherichia coli
The phytochemical analysis and antibacterial activity of methanolic and ethanolic leaf and stem bark extracts of baobab tree on Escherichia coli and Staphylococcus aureus were carried out using agar well diffusion method...