Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydın in Turkey
Journal Title: Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi - Year 2018, Vol 6, Issue 1
Abstract
The aim of the present study was to identify the haplotypes of the Varroa destructor mite which infects honeybees (Apis mellifera) in the province of Aydın in Turkey, using two different modified techniques for the mitochondrial Cox1 gene of the mite. In order to confirm the haplotype, two different primer pairs were selected. 376 bp DNA in size was amplified using the first primer pair. SacI restriction enzyme was applied to the amplified products; however, this restriction enzyme did not cut the DNA. 570 bp DNA in size was amplified using the second primer pair. XhoI and SacI restriction enzymes were used for the amplified products. Although, the SacI restriction enzyme did not cut the DNA, the XhoI restriction enzyme cut the amplified DNA into two fragments (bands), with the sizes of 270 and 300 bp two bands 270 and 300 bp. While comparing the results, these bands were found specific for Korean haplotype of V. destructor. In conclusion, all of the 200 samples of V. destructor examined in this study were identified to be the Korean haplotype
Authors and Affiliations
Adnan Ayan, Osman Selçuk Aldemir
Keywords
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- EP ID EP428044
- DOI 10.24998/maeusabed.435383
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