Genetic Diversity of Ethiopian Durum Wheat (Triticum durum Desf.) Landrace Collections as Reveled by SSR Markers
Journal Title: Advances in Crop Science and Technology - Year 2019, Vol 7, Issue 1
Abstract
The genetic diversity of 141 Ethiopian durum wheat (Triticum durum Desf.) landrace collections and 19 improved varieties was analyzed using primers of 12 SSR markers in 2015. The experiment was conducted at the National Agricultural Biotechnology Laboratory, Holetta, Ethiopia. The difference between the longest and shortest amplified fragment size ranged from 100 to 350 bp. The highest variation in fragment size was observed for primer CFD 257 (250-350 bp) and the lowest was for primer CFA2278 (100-180 bp). All the 12 SSR primer pairs were polymorphic and generated a total of 74 alleles. The number of alleles per locus ranged from 4 for primers WMS 375, WMS 493, and CFD257 to 9 for primers WMS53, with a mean of 6 alleles per locus. Some loci were more monomorphic (WMS493, WMS516, and WMS532) with less gene flow than other loci such as WMS269 and WMS234. Based on the analysis of molecular variance (AMOVA), lower level (19%) of variation was observed among populations and higher level (81%) within population. The dendogram of cluster analysis based on neighbor-joining algorithm categorized the 160 durum wheat genotypes into three major clusters. Cluster I consisted 47, Cluster II 64, and Cluster III 49 genotypes. Based on the magnitude of the genetic distance (GD), more differentiations were observed between accessions in populations originated from different geographical regions in Ethiopia.
Authors and Affiliations
Meseret Asmamaw, Gemechu Keneni, Kassahun Tesfaye
Keywords
Related Articles
- EP ID EP630485
- DOI -
- Views 64
- Downloads 0
Evaluation of Bread Wheat (Triticum aestivum L.) Genotypes for Yield Potential and Related Traits Under High Temperature Stress Condition at Middle Awash, Ethiopia
Information on the extent and performance of genotypes present in a crop species under high temperature stress are essential for effective crop adaptation program. To generate such information 36 bread wheat genotypes wi...
Genetic Diversity of Ethiopian Durum Wheat (Triticum durum Desf.) Landrace Collections as Reveled by SSR Markers
The genetic diversity of 141 Ethiopian durum wheat (Triticum durum Desf.) landrace collections and 19 improved varieties was analyzed using primers of 12 SSR markers in 2015. The experiment was conducted at the National...
Decade of South Ethiopian Coffee Improvement Activities at Awada Coffee Research Center
Coffee improvement program of Ethiopia was aimed to develop widely adaptable and stable cultivars across all coffee growing regions of the country although there is a significant ecological variation that prevails betwee...
Soil Test and Tissue Analysis Based Nitrogen and Phosphorus Fertilizer Applications on Teff [Eragrostis tef (Zucc.) Trotter] Cultivars in North Shewa Central Highlands, Ethiopia
Soil fertility studies and crop improvement have brought remarkable change in crop production particularly in teff in Ethiopia. Differences in soil status affect productivities of various cultivars in which their nutriti...
Impact of Zinc and Organic Manure on Wheat Productivity and Soil Fertility
Use of synthetic fertilizers have some problem to farmer due to short residual effect and high cost. Since the farmers used organic manure due to long residual effect but the nutritional requirement is not fulfilling dur...