Genetic markers assisted selection in cattle: leptine gene locus and its polymorphism.
Journal Title: Animal Biology & Animal Husbandry - International Journal of the Bioflux Society - Year 2009, Vol 1, Issue 1
Abstract
We studied the polymorphism of types RFLP-Sau 3AI, RFLP-Mbo I and RFLP-Bsa AI at the leptin gene locus, in two improved genuine breeds: Baltata Romaneasca and Bruna de Maramurea. The comparative use of different DNA separation, amplification and genotypization protocols allowed us to select the most suitable techniques for polymorphism studies, taking into consideration the accuracy of the obtained results, reproducibility, costs and equipments. The DNA obtained using automatic MagnaPure LC kit, was of a very good quality, and did not exhibit traces of degradation. It iwas free of RNA and protein contamination. The posibility of using refrigerated and old blood advantages the use of this method. We recommend the use of protocols described by Liefers et al (2002) and Lien et al (1997) for the study of polymorphism of the leptin gene and genotypization at this locus, as we demonstrated that they could produce better results, as compared to those obtained when the protocol of Pomp et al (1997) was used. In the studied Baltata Romaneasca and Bruna de Maramures populations, the allele A had a higher frequency at the leptin gene locus, as compared to allele B. Analyzing the frequencies of the genotypes in Baltata Romaneasca individuals, we noticed that the AA homozygous genotype represents 60%, AB heterozygous genotype 36% and BB homozygous genotype the smallest share, 4%, respectively of the total genotypes. Frequencies were similar for Bruna de Maramures, with the difference that BB genotype was absent.
Authors and Affiliations
Teodora-Crina Carsai
Keywords
Related Articles
- EP ID EP97001
- DOI -
- Views 189
- Downloads 0
Potential molecular biomarkers of crowding stress in Atlantic cod, Gadus morhua and their importance in health management
Sub-adult Atlantic cod, Gadus morhua were subjected to short-term crowding stress in order to map out potential biomarkers of stress response of this fish at the molecular level. This short review summarizes the results...
Polyculture of white shrimp, Litopenaeus vannamei and milkfish, Chanos chanos as a strategy for efficient utilization of natural food production in ponds
Both shrimps and milkfish are important aquaculture species in the Philippines. In the present study, we described a modified extensive polyculture method for white shrimp, Litopenaeus vannamei and miklkfish, Chanos chan...
Physicochemical and microbiological research on characteristics of meat products during storage in the membrane depending on the quality of raw materials.
In our research we followed changes in the organoleptic, physicochemical and microbiological characteristics of meat products within the warranty period depending on the quality of raw materials and storage conditions. I...
Research on raw material correlation and soy protein derivatives used for meat preparations in membrane.
Since the Romanian literature is pretty low quality data on the influence of hygienic quality of raw materials and preparations of meat, in our research we intend to pursue these issues in pork and beef quality I and II....
Contributions to the knowledge of the Romanian from the western Dacian Basin: The molluscs’ fauna from Răcarii de Jos (Dolj County).
In the western Dacian Basin, the Romanian age starts with a fauna comprised of carved unionidae and robust viviparidae, which appear in numerous crops in Mehedinţi, Dolj and Gorj Counties. The lamellibranchiate species f...