GENETIC POLYMORPHISM OF LONG-CLAWED CRAYFISH (Astacus leptodactylus Esch.) IN THE LAKES OF THE BELARUS POLESIA
Journal Title: Водні біоресурси та аквакультура - Year 2018, Vol 1, Issue
Abstract
The article presents the results of the work on genotyping and the study of the genetic polymorphism of long-clawed crayfish (Astacus leptodactylus Esch.) living in the lakes of Oltush and Sominsk, Brest region. Two mitochondrial genes, COI and 16s rRNA, has been used. When assessing the species of crayfish by the COI mtDNA gene, it was found that in both lakes, with a probability of 99%, there was a species of decapod crayfish – a long-clawed crayfish (Astacus leptodactylus Esch.). In the course of the research, the technique of the lifetime selection of biological material in long-clawed crayfish (Astacus leptodactylus Esch.) was adapted, and the method for isolating DNA from the muscle tissue of long-clawed crayfish was improved. For the first time in the country, a bank of DNA and biological samples of long- clawed crayfish has been created. The work was performed as part of task 2.11 “Study of the genetic diversity of aquatic and near-water invertebrates” of the state program of scientific research “Biotechnology” 2016-2020, the subprogram “Structural and functional Genomics”.
Authors and Affiliations
М. О. Сасіновіч, О. М. Слуквін, А. В. Алехнович
Keywords
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- EP ID EP589552
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